吸附
扫描电子显微镜
化学
化学工程
十二烷基硫酸钠
动力学
骨料(复合)
聚丙烯酰胺凝胶电泳
色谱法
材料科学
纳米技术
生物化学
有机化学
复合材料
量子力学
物理
工程类
酶
作者
Wenqiang Cai,Bowen Zou,Xiaokang Na,Chao Ren,Xiaohan Zheng,Xianbing Xu,Ming Du,Beiwei Zhu,Chao Wu
标识
DOI:10.1016/j.foodchem.2024.140818
摘要
The effects of adsorption behavior and assembly mechanism of proteins and lipids at the interface on the formation of yuba films were investigated. The thickness of yuba films increased rapidly from nano to micro scale within minutes according to the scanning electron microscopy (SEM) images. The confocal laser scanning microscope (CLSM), SEM images, and sodium dodecyl sulfate-polyacrylamide gel electrophoresis revealed that the formation of protein aggregates (40-100 nm) was an essential requirement for the development of yuba. Meanwhile, a relatively loose spatial structure was formed by protein aggregates under the influence of water vapor. This structure served as the foundation for incorporating lipids. Interfacial adsorption kinetics indicated that increasing the concentration (from 3 to 9 mg/mL) of protein aggregates enhanced the rearrangement rate. This finding demonstrated that the variations of interfacial protein aggregate concentration were a crucial factor leading to the non-linear growth of film thickness.
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