A benzothiazole-salt-based fluorescent probe for precise monitoring the changes of pH and viscosity

This work presented a novel ratiometric fluorescent probe (NBO) based on benzothiazole dye, which could monitor the pH fluctuations with high sensitivity via the intramolecular charge transfer (ICT) process. NBO was developed with a good linear response in the pH range of 5.75–7.00 (pKa = 6.5) and a reversible structural change in acidic and alkaline environments. Besides, NBO also has the potential to detect the viscosity changes. Meanwhile, NBO has been successfully applied to the pH monitoring of a variety of water samples in natural environment and human serum. With the treatment of different solutions at pH 2.0 - pH 9.0, the test strips showed significant color changes under both 365 nm UV lamp and room light. When the test strips were applied to white wine, pH could be detected quickly and easily by the naked eyes. Therefore, a novel probe that can be used to detect pH in environment, human serum and food has been successfully developed.