化学
重复性
DNA
核酸内切酶
聚合酶链反应
限制性酶
分子生物学
检出限
转化(遗传学)
模板
色谱法
线性范围
实时聚合酶链反应
前列腺特异性抗原
前列腺癌
生物化学
基因
癌症
遗传学
生物
材料科学
纳米技术
作者
Li Zhao,Jing‐jing Fu,Li Wang,Yingzhu Zhou,Jinyan Li,Shengbin He
出处
期刊:Talanta
[Elsevier]
日期:2024-01-01
卷期号:267: 125206-125206
标识
DOI:10.1016/j.talanta.2023.125206
摘要
A switch DNA template was designed to transform proteins into linear DNA strands of 97 nt. The linear DNA was subjected to quantitative polymerase chain reaction (qPCR) to determine the initial copy number, which correlated positively with the protein concentration. A restriction endonuclease was used to remove background amplification. Using prostate-specific antigen (PSA) as a protein model, the established method quantified PSA in the range of 10−18-10−13 mol/mL (detection limit = 0.034 pg/mL) with an R2 of 0.974. Good repeatability and specificity of the method were demonstrated. The established method was successfully applied for the quantification of serum PSA levels in women. Significant differences in PSA levels were observed between healthy participants and polycystic ovary syndrome patients.
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