The impact of microfluidics sperm processing on blastocyst euploidy rates compared with density gradient centrifugation: a sibling oocyte double-blinded prospective randomized clinical trial

胚泡 卵母细胞 男科 密度梯度 精子 医学 妇科 生物 胚胎 遗传学 物理 胚胎发生 量子力学
作者
Prachi N. Godiwala,Jane Kwieraga,Emilse Almanza,Evelyn Neuber,Daniel R. Grow,Claudio Benadiva,Reeva Makhijani,Andrea DiLuigi,David Schmidt,Alison Bartolucci,Lawrence Engmann
出处
期刊:Fertility and Sterility [Elsevier BV]
卷期号:122 (1): 85-94 被引量:5
标识
DOI:10.1016/j.fertnstert.2024.02.021
摘要

Abstract

Objective

To compare euploidy rates among blastocysts created from sibling oocytes injected with sperm processed by microfluidics or by density gradient centrifugation.

Design

Sibling oocyte randomized controlled trial.

Setting

Single university-affiliated infertility practice.

Patient(s)

106 patients aged 18-42 undergoing fresh in vitro fertilization (IVF) cycles with preimplantation genetic testing (PGT) between January 2021 to April 2022 contributed 1442 mature oocytes which were injected with sperm processed via microfluidics or density gradient centrifugation.

Intervention(s)

Sperm sample divided and processed via a microfluidics device and density gradient centrifugation for injection into sibling oocytes.

Main outcome measure(s)

Primary outcome was the embryo euploidy rate. Secondary outcomes included the fertilization, high quality blastulation, and ongoing pregnancy rates.

Result(s)

The blastocyst euploidy rate per mature oocyte was not significantly different in the study group compared to the control group (22.9% versus 20.5%, p=0.60). The blastocyst euploidy rate per biopsied embryo was also similar between the two groups (53.0% versus 45.7%, p=0.34). However, the fertilization rate per mature oocyte injected was found to be significantly higher in the study group compared to the control group (76.0% versus 69.9%, p=0.03). The high quality blastulation rate per mature oocyte injected was similar between the two groups, as was the total number of embryos frozen. There were no differences in the number of participants with no blastocysts for biopsy or the number of participants with no euploid embryos between the two groups. Among the male factor infertility and recurrent pregnancy loss subgroups, there were no differences in euploidy rates, fertilization rates, blastulation rates, or total numbers of blastocysts frozen, although the study was underpowered to detect these differences. Seventy-seven patients underwent frozen embryo transfer; there were no significant differences in pregnancy outcomes between the two groups.

Conclusion(s)

Microfluidics processing did not improve embryo euploidy rates compared to density gradient centrifugation in this sibling oocyte study, although fertilization rates were significantly higher.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
PDF的下载单位、IP信息已删除 (2025-6-4)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
小彭友完成签到,获得积分10
6秒前
7秒前
josie完成签到 ,获得积分10
11秒前
llll完成签到 ,获得积分10
11秒前
量子星尘发布了新的文献求助10
11秒前
韭菜发布了新的文献求助10
11秒前
外向的斑马完成签到 ,获得积分10
12秒前
村长热爱美丽完成签到 ,获得积分10
14秒前
尹尹关注了科研通微信公众号
16秒前
呆呆完成签到 ,获得积分10
17秒前
xianyaoz完成签到 ,获得积分0
24秒前
杨远杰完成签到,获得积分10
25秒前
蓝桉完成签到 ,获得积分10
25秒前
JuliaWang完成签到 ,获得积分10
32秒前
无限的含羞草完成签到,获得积分10
33秒前
八二力完成签到 ,获得积分10
37秒前
韭菜发布了新的文献求助10
40秒前
情怀应助科研通管家采纳,获得30
43秒前
water应助科研通管家采纳,获得10
43秒前
JamesPei应助科研通管家采纳,获得10
43秒前
2012csc完成签到 ,获得积分0
45秒前
风清扬应助韭菜采纳,获得10
46秒前
WSY完成签到 ,获得积分10
47秒前
虞无声发布了新的文献求助10
48秒前
执着新蕾完成签到,获得积分10
50秒前
Vivian完成签到 ,获得积分10
53秒前
666完成签到 ,获得积分10
55秒前
1分钟前
量子星尘发布了新的文献求助10
1分钟前
蔡从安完成签到,获得积分10
1分钟前
奥雷里亚诺完成签到 ,获得积分10
1分钟前
不呆完成签到 ,获得积分10
1分钟前
Cheung2121发布了新的文献求助30
1分钟前
画龙完成签到,获得积分10
1分钟前
韭菜完成签到,获得积分20
1分钟前
Owen应助Cheung2121采纳,获得10
1分钟前
爱学习的小李完成签到 ,获得积分10
1分钟前
若水完成签到 ,获得积分10
1分钟前
SYLH应助tian采纳,获得10
1分钟前
脑洞疼应助tian采纳,获得10
1分钟前
高分求助中
【提示信息,请勿应助】关于scihub 10000
Les Mantodea de Guyane: Insecta, Polyneoptera [The Mantids of French Guiana] 3000
徐淮辽南地区新元古代叠层石及生物地层 3000
The Mother of All Tableaux: Order, Equivalence, and Geometry in the Large-scale Structure of Optimality Theory 3000
Handbook of Industrial Diamonds.Vol2 1100
Global Eyelash Assessment scale (GEA) 1000
Picture Books with Same-sex Parented Families: Unintentional Censorship 550
热门求助领域 (近24小时)
化学 材料科学 医学 生物 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 遗传学 基因 物理化学 催化作用 冶金 细胞生物学 免疫学
热门帖子
关注 科研通微信公众号,转发送积分 4038112
求助须知:如何正确求助?哪些是违规求助? 3575788
关于积分的说明 11373801
捐赠科研通 3305604
什么是DOI,文献DOI怎么找? 1819255
邀请新用户注册赠送积分活动 892655
科研通“疑难数据库(出版商)”最低求助积分说明 815022