The impact of microfluidics sperm processing on blastocyst euploidy rates compared with density gradient centrifugation: a sibling oocyte double-blinded prospective randomized clinical trial

胚泡 卵母细胞 男科 密度梯度 精子 医学 妇科 生物 胚胎 遗传学 物理 胚胎发生 量子力学
作者
Prachi N. Godiwala,Jane Kwieraga,Emilse Almanza,Evelyn Neuber,Daniel R. Grow,Claudio Benadiva,Reeva Makhijani,Andrea DiLuigi,David Schmidt,Alison Bartolucci,Lawrence Engmann
出处
期刊:Fertility and Sterility [Elsevier BV]
卷期号:122 (1): 85-94 被引量:5
标识
DOI:10.1016/j.fertnstert.2024.02.021
摘要

Abstract

Objective

To compare euploidy rates among blastocysts created from sibling oocytes injected with sperm processed by microfluidics or by density gradient centrifugation.

Design

Sibling oocyte randomized controlled trial.

Setting

Single university-affiliated infertility practice.

Patient(s)

106 patients aged 18-42 undergoing fresh in vitro fertilization (IVF) cycles with preimplantation genetic testing (PGT) between January 2021 to April 2022 contributed 1442 mature oocytes which were injected with sperm processed via microfluidics or density gradient centrifugation.

Intervention(s)

Sperm sample divided and processed via a microfluidics device and density gradient centrifugation for injection into sibling oocytes.

Main outcome measure(s)

Primary outcome was the embryo euploidy rate. Secondary outcomes included the fertilization, high quality blastulation, and ongoing pregnancy rates.

Result(s)

The blastocyst euploidy rate per mature oocyte was not significantly different in the study group compared to the control group (22.9% versus 20.5%, p=0.60). The blastocyst euploidy rate per biopsied embryo was also similar between the two groups (53.0% versus 45.7%, p=0.34). However, the fertilization rate per mature oocyte injected was found to be significantly higher in the study group compared to the control group (76.0% versus 69.9%, p=0.03). The high quality blastulation rate per mature oocyte injected was similar between the two groups, as was the total number of embryos frozen. There were no differences in the number of participants with no blastocysts for biopsy or the number of participants with no euploid embryos between the two groups. Among the male factor infertility and recurrent pregnancy loss subgroups, there were no differences in euploidy rates, fertilization rates, blastulation rates, or total numbers of blastocysts frozen, although the study was underpowered to detect these differences. Seventy-seven patients underwent frozen embryo transfer; there were no significant differences in pregnancy outcomes between the two groups.

Conclusion(s)

Microfluidics processing did not improve embryo euploidy rates compared to density gradient centrifugation in this sibling oocyte study, although fertilization rates were significantly higher.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
PDF的下载单位、IP信息已删除 (2025-6-4)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
2秒前
朴实山兰完成签到,获得积分10
4秒前
6秒前
SciGPT应助ylky采纳,获得10
6秒前
小爪冰凉发布了新的文献求助10
6秒前
7秒前
量子星尘发布了新的文献求助10
7秒前
JamesPei应助科研小牛采纳,获得150
8秒前
Ava应助科研小牛采纳,获得10
8秒前
8秒前
忧郁盼夏发布了新的文献求助10
11秒前
marina关注了科研通微信公众号
11秒前
沐紫心完成签到 ,获得积分10
12秒前
科研通AI5应助suger采纳,获得10
12秒前
14秒前
八九发布了新的文献求助10
14秒前
17秒前
17秒前
一个好听的名字完成签到,获得积分10
18秒前
wbh发布了新的文献求助20
18秒前
18秒前
19秒前
Eddoes完成签到,获得积分10
19秒前
WN发布了新的文献求助10
20秒前
诚心的凌旋完成签到,获得积分10
20秒前
leanne发布了新的文献求助10
22秒前
机智思真完成签到,获得积分10
22秒前
23秒前
科研小牛完成签到,获得积分10
23秒前
归尘发布了新的文献求助10
23秒前
万物安生发布了新的文献求助10
23秒前
阿钉发布了新的文献求助10
23秒前
24秒前
张钦奎发布了新的文献求助10
24秒前
24秒前
香蕉觅云应助文献采纳,获得30
25秒前
25秒前
彭栋应助魁梧的鲂采纳,获得10
26秒前
明道若昧发布了新的文献求助10
28秒前
30秒前
高分求助中
A new approach to the extrapolation of accelerated life test data 1000
ACSM’s Guidelines for Exercise Testing and Prescription, 12th edition 500
‘Unruly’ Children: Historical Fieldnotes and Learning Morality in a Taiwan Village (New Departures in Anthropology) 400
Indomethacinのヒトにおける経皮吸収 400
Phylogenetic study of the order Polydesmida (Myriapoda: Diplopoda) 370
基于可调谐半导体激光吸收光谱技术泄漏气体检测系统的研究 350
Robot-supported joining of reinforcement textiles with one-sided sewing heads 320
热门求助领域 (近24小时)
化学 材料科学 医学 生物 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 遗传学 基因 物理化学 催化作用 冶金 细胞生物学 免疫学
热门帖子
关注 科研通微信公众号,转发送积分 3989378
求助须知:如何正确求助?哪些是违规求助? 3531442
关于积分的说明 11254002
捐赠科研通 3270126
什么是DOI,文献DOI怎么找? 1804887
邀请新用户注册赠送积分活动 882087
科研通“疑难数据库(出版商)”最低求助积分说明 809173