核糖核酸
清脆的
引导RNA
计算生物学
小RNA
RNA干扰
合成生物学
亚基因组mRNA
RNA沉默
计算机科学
Cas9
生物
基因
遗传学
作者
Guo Jiang,Yuanli Gao,Nan Zhou,Baojun Wang
标识
DOI:10.1016/j.tibtech.2024.04.002
摘要
RNA sensing in vivo evaluates past or ongoing endogenous RNA disturbances, which is crucial for identifying cell types and states and diagnosing diseases. Recently, the CRISPR-driven genetic circuits have offered promising solutions to burgeoning challenges in RNA sensing. This review delves into the cutting-edge developments of CRISPR-powered RNA sensors in vivo, reclassifying these RNA sensors into four categories based on their working mechanisms, including programmable reassembly of split single-guide RNA (sgRNA), RNA-triggered RNA processing and protein cleavage, miRNA-triggered RNA interference (RNAi), and strand displacement reactions. Then, we discuss the advantages and challenges of existing methodologies in diverse application scenarios and anticipate and analyze obstacles and opportunities in forthcoming practical implementations.
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