清晨好,您是今天最早来到科研通的研友!由于当前在线用户较少,发布求助请尽量完整地填写文献信息,科研通机器人24小时在线,伴您科研之路漫漫前行!

Two-dimensional design strategy to construct smart dual-responsive fluorescent probe for the precise tracking of ischemic stroke

构造(python库) 对偶(语法数字) 跟踪(教育) 缺血性中风 荧光 计算机科学 冲程(发动机) 医学 工程类 心理学 内科学 缺血 物理 计算机网络 机械工程 光学 文学类 艺术 教育学
作者
Jiayu Zeng,Minhui Liu,Ting Yang,Jia Huang,Songjiao Li,Wanting Zhang,Dan Cheng,Longwei He,Jia Zhou
出处
期刊:Chinese Chemical Letters [Elsevier BV]
卷期号:: 110166-110166 被引量:2
标识
DOI:10.1016/j.cclet.2024.110166
摘要

Early recognition is key to improving the prognosis of ischemic stroke (IS), while available imaging methods tend to target events that have already undergone ischemia. A new method to detect early IS is urgently needed, as well as further study of its mechanisms. Viscosity and cysteine (Cys) levels of mitochondria have been associated with ferroptosis and IS. It is possible to identify IS and ferroptosis accurately and early by monitoring changes in mitochondrial Cys and viscosity simultaneously. In this work, a viscosity/Cys dual-responsive mitochondrial-targeted near-infrared (NIR) fluorescent probe (NVCP) was constructed for the precise tracking of IS using a two-dimensional design strategy. NVCP consists of a chromophore dyad containing diethylaminostyrene quinolinium rotor and chloro-sulfonylbenzoxadiazole (SBD-Cl) derivative with two easily distinguished emission bands (λem = 592 and 670 nm). NVCP performs the way of killing two birds with one stone, that is, the probe exhibits excellent selectivity and sensitivity for detecting viscosity and Cys in living cells with excellent biocompatibility and accurate mitochondrial targeting capability by dual channel imaging mode. In addition, NVCP recognized that the viscosity increases and Cys level decreases in cells when undergoing ferroptosis and oxygen-glucose deprivation (OGD) stress by confocal imaging, flow cytometry, and Western blot experiments. Treatment of ferroptosis inhibitors (ferrostatin-1 (Fer-1) and deferoxamine (DFO)) could reverse the variation tendency of viscosity and Cys. This is the first time that the relationship between ferroptosis and IS was identified through an analysis of Cys and viscosity. More importantly, the ischemic area was also instantly distinguished from normal tissues through fluorescence imaging of NVCP in vivo. The developed NIR dual-responsive probe NVCP toward viscosity and Cys could serve as a sensitive and reliable tool for tracking ferroptosis-related pathological processes during IS.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
PDF的下载单位、IP信息已删除 (2025-6-4)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
啦啦啦完成签到 ,获得积分10
2秒前
无悔完成签到 ,获得积分10
22秒前
量子星尘发布了新的文献求助10
39秒前
完美世界应助科研通管家采纳,获得10
40秒前
SciGPT应助科研通管家采纳,获得10
40秒前
握瑾怀瑜完成签到 ,获得积分0
54秒前
笨笨青筠完成签到 ,获得积分10
58秒前
dexrer完成签到,获得积分10
1分钟前
傻傻的念梦关注了科研通微信公众号
1分钟前
5433完成签到 ,获得积分10
1分钟前
1分钟前
1分钟前
滴滴滴完成签到 ,获得积分10
1分钟前
量子星尘发布了新的文献求助10
2分钟前
坦率雪枫完成签到 ,获得积分10
2分钟前
Skywings完成签到,获得积分10
2分钟前
2分钟前
3分钟前
默默雪旋完成签到 ,获得积分10
3分钟前
量子星尘发布了新的文献求助10
3分钟前
4分钟前
k001boyxw完成签到,获得积分10
4分钟前
ChatGPT完成签到,获得积分10
4分钟前
4分钟前
量子星尘发布了新的文献求助10
4分钟前
科研通AI2S应助科研通管家采纳,获得10
4分钟前
4分钟前
雪白小丸子完成签到,获得积分10
4分钟前
5分钟前
ch发布了新的文献求助10
5分钟前
lulululululu完成签到,获得积分20
5分钟前
李志全完成签到 ,获得积分10
5分钟前
量子星尘发布了新的文献求助10
5分钟前
6分钟前
6分钟前
6分钟前
调皮金连发布了新的文献求助10
6分钟前
深情安青应助科研通管家采纳,获得10
6分钟前
6分钟前
之_ZH完成签到 ,获得积分10
7分钟前
高分求助中
A new approach to the extrapolation of accelerated life test data 1000
Picture Books with Same-sex Parented Families: Unintentional Censorship 700
ACSM’s Guidelines for Exercise Testing and Prescription, 12th edition 500
Nucleophilic substitution in azasydnone-modified dinitroanisoles 500
不知道标题是什么 500
Indomethacinのヒトにおける経皮吸収 400
Phylogenetic study of the order Polydesmida (Myriapoda: Diplopoda) 370
热门求助领域 (近24小时)
化学 材料科学 医学 生物 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 遗传学 基因 物理化学 催化作用 冶金 细胞生物学 免疫学
热门帖子
关注 科研通微信公众号,转发送积分 3976683
求助须知:如何正确求助?哪些是违规求助? 3520788
关于积分的说明 11204819
捐赠科研通 3257565
什么是DOI,文献DOI怎么找? 1798756
邀请新用户注册赠送积分活动 877897
科研通“疑难数据库(出版商)”最低求助积分说明 806648