球体
显微镜
薄层荧光显微镜
材料科学
葡萄孢霉素
三维细胞培养
生物医学工程
光学
吞吐量
化学
细胞培养
扫描共焦电子显微镜
细胞
计算机科学
生物
医学
酶
生物化学
遗传学
蛋白激酶C
无线
物理
电信
作者
Tingting Zhu,Jun Nie,Tingting Yu,Dan Zhu,Yanyi Huang,Zaozao Chen,Zhongze Gu,Jiang Tang,Dongyu Li,Peng Fei
摘要
Light sheet microscopy combined with a microchip is an emerging tool in biomedical research that notably improves efficiency. However, microchip-enhanced light-sheet microscopy is limited by noticeable aberrations induced by the complex refractive indices in the chip. Herein, we report a droplet microchip that is specifically engineered to be capable of large-scale culture of 3D spheroids (over 600 samples per chip) and has a polymer index matched to water (difference <1%). When combined with a lab-built open-top light-sheet microscope, this microchip-enhanced microscopy technique allows 3D time-lapse imaging of the cultivated spheroids with ∼2.5-µm single-cell resolution and a high throughput of ∼120 spheroids per minute. This technique was validated by a comparative study on the proliferation and apoptosis rates of hundreds of spheroids with or without treatment with the apoptosis-inducing drug Staurosporine.
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