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Rapid FRET-based homogeneous immunoassay of procalcitonin using matched carbon dots labels

降钙素原 检出限 材料科学 荧光 同种类的 费斯特共振能量转移 碳纤维 分析化学(期刊) 碳纳米颗粒 发光 免疫分析 激发 线性范围 纳米颗粒 纳米技术 抗体 化学 光电子学 色谱法 医学 光学 免疫学 复合数 工程类 复合材料 热力学 物理 电气工程 败血症
作者
Bo Liu,Kun Yang,Siyu Lu,Junjie Cai,Fan Li,Feng Tian
出处
期刊:Nanotechnology [IOP Publishing]
卷期号:33 (8): 085702-085702 被引量:4
标识
DOI:10.1088/1361-6528/ac3aab
摘要

A novel method for the detection of procalcitonin in a homogeneous system by matched carbon dots (CDs) labeled immunoprobes was proposed based on the principle of FRET and double antibody sandwich method. Blue-emitting carbon dots with a strong fluorescence emission range of 400-550 nm and red-emitting carbon dots with the best excitation range of 410-550 nm were prepared before they reacted with procalcitonin protoclone antibody pairs to form immunoprobes. According to the principles of FRET, blue-emitting carbon dots were selected as the energy donor and red-emitting carbon dots as the energy receptor. The external light source excitation (310 nm) could only cause weak luminescence of CDs. However, once procalcitonin was added, procalcitonin and antibodies would be combined with each other quickly (≤20 min). Here, blue-emitting carbon dots acquired energy could be transferred to red-emitting carbon dots efficiently, causing the emitted fluorescence enhancement of red-emitting carbon dots. The fluorescence detection results in PBS buffer solution and diluted rabbit blood serum showed that the fluorescence intensity variation was linear with the concentration of procalcitonin. There was a good linear relationship betweenF/F0 and procalcitonin concentrations in PBS buffer solution that ranged from 0 to 100 ng ml-1, and the linear equation wasF/F0 = 0.004 *Cpct + 0.98359. Detection in the diluted rabbit serum led to the results that were linear in two concentration ranges, including 0-40 ng ml-1and 40-100 ng ml-1, and the detection limit based on 3σK-1was 0.52 ng ml-1. It is likely that this matched CDs labeled immunoprobes system can provide a new mode for rapid homogeneous detection of disease markers.
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