Banxia xiexin decoction affects drug sensitivity in gastric cancer cells by regulating MGMT expression via IL‑6/JAK/STAT3‑mediated PD‑L1 activity

癌症研究 生物 流式细胞术 免疫印迹 癌症 甲基转移酶 细胞凋亡 细胞周期 车站3 癌细胞 癌基因 分子生物学 甲基化 基因 生物化学 遗传学
作者
Xuan Feng,Feng Xue,Guihua He,Qing Ni,Suiping Huang
出处
期刊:International Journal of Molecular Medicine [Spandidos Publishing]
卷期号:48 (2) 被引量:19
标识
DOI:10.3892/ijmm.2021.4998
摘要

Banxia xiexin decoction (BXXX) is a classic preparation used to treat gastrointestinal diseases, and also has certain therapeutic effects on gastrointestinal tumors. BXXX has been reported to regulate the expression of proteins associated with drug resistance and sensitivity in tumors, and thus, the aim of the present study was to investigate the mechanisms of BXXX drug sensitivity in gastric cancer (GC). The expression levels of programmed cell death 1 ligand 1 (PD‑L1), 6‑O‑methylguanine‑DNA methyltransferase (MGMT) and STAT3 were immunohistochemically detected in the cancer and adjacent non‑cancer tissues of patients with GC, and in vitro experimentation was conducted using drug‑resistant and ‑sensitive GC cells. The expression levels of PD‑L1, MGMT and STAT3 were determined using reverse transcription‑quantitative PCR. Different concentrations of BXXX drug serum were used to treat the cells and the cellular inhibition rate was assessed using a Cell Counting Kit‑8 assay. Flow cytometry was used to detect apoptosis, and western blot analysis was used to detect the expression levels of IL‑6, IFN‑γ, JAK/STAT3 pathway proteins, PD‑L1 and MGMT. The association between PD‑L1 and MGMT protein expression levels was subsequently assessed via co‑immunoprecipitation. Furthermore, in vivo studies were conducted following the establishment of a drug‑resistant tumor‑bearing mouse model, where GC tumor size was assessed under different treatment conditions, and western blot analysis was used to detect the expression of related pathway proteins. The expression levels of PD‑L1, MGMT and STAT3 were significantly increased in GC tissues, GC cells and cisplatin‑resistant cells. Furthermore, BXXX inhibited the proliferation of drug‑resistant cells and promoted the inhibitory effects of chemotherapeutic drugs on drug‑resistant cells. BXXX also inhibited the expression levels of IL‑6, IFN‑γ and JAK/STAT3 pathway proteins, as well as the expression levels of PD‑L1 and MGMT. Colivelin, an activator of STAT3, reversed the effects of BXXX on drug‑resistant GC cells, and significantly reversed the effect of BXXX on PD‑L1 expression. In conclusion, BXXX was found to influence the drug sensitivity of GC cells by regulating the expression of MGMT. This process functions viaPD‑L1, which was itself mediated by IL‑6/JAK/STAT3 signaling.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
PDF的下载单位、IP信息已删除 (2025-6-4)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
刚刚
睡到自然醒完成签到 ,获得积分10
1秒前
cis2014完成签到,获得积分10
3秒前
独特的大有完成签到 ,获得积分10
3秒前
量子星尘发布了新的文献求助10
4秒前
6秒前
xingyi完成签到,获得积分10
8秒前
9秒前
舒心的秋荷完成签到 ,获得积分10
12秒前
zz123发布了新的文献求助10
13秒前
liaomr完成签到 ,获得积分10
13秒前
粗犷的灵松完成签到,获得积分10
14秒前
吃小孩的妖怪完成签到 ,获得积分10
14秒前
ncuwzq完成签到,获得积分10
16秒前
yshj完成签到 ,获得积分10
17秒前
19秒前
净禅完成签到 ,获得积分10
21秒前
23秒前
迷人的寒风完成签到,获得积分10
24秒前
24秒前
water应助科研通管家采纳,获得10
25秒前
Lucas应助HHHAN采纳,获得10
27秒前
无情修杰完成签到 ,获得积分10
28秒前
小柒完成签到 ,获得积分10
30秒前
聪慧芷巧发布了新的文献求助10
31秒前
32秒前
36秒前
蓝意完成签到,获得积分0
37秒前
xiaohongmao完成签到,获得积分10
42秒前
45秒前
qweerrtt完成签到,获得积分10
52秒前
52秒前
与共发布了新的文献求助10
53秒前
carly完成签到 ,获得积分10
54秒前
颢懿完成签到 ,获得积分10
57秒前
量子星尘发布了新的文献求助10
58秒前
1分钟前
ljc完成签到 ,获得积分10
1分钟前
Java完成签到,获得积分10
1分钟前
1分钟前
高分求助中
【提示信息,请勿应助】关于scihub 10000
Les Mantodea de Guyane: Insecta, Polyneoptera [The Mantids of French Guiana] 3000
徐淮辽南地区新元古代叠层石及生物地层 3000
The Mother of All Tableaux: Order, Equivalence, and Geometry in the Large-scale Structure of Optimality Theory 3000
Handbook of Industrial Diamonds.Vol2 1100
Global Eyelash Assessment scale (GEA) 1000
Picture Books with Same-sex Parented Families: Unintentional Censorship 550
热门求助领域 (近24小时)
化学 材料科学 医学 生物 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 遗传学 基因 物理化学 催化作用 冶金 细胞生物学 免疫学
热门帖子
关注 科研通微信公众号,转发送积分 4038066
求助须知:如何正确求助?哪些是违规求助? 3575779
关于积分的说明 11373801
捐赠科研通 3305584
什么是DOI,文献DOI怎么找? 1819239
邀请新用户注册赠送积分活动 892655
科研通“疑难数据库(出版商)”最低求助积分说明 815022