亲爱的研友该休息了!由于当前在线用户较少,发布求助请尽量完整地填写文献信息,科研通机器人24小时在线,伴您度过漫漫科研夜!身体可是革命的本钱,早点休息,好梦!

Banxia xiexin decoction affects drug sensitivity in gastric cancer cells by regulating MGMT expression via IL‑6/JAK/STAT3‑mediated PD‑L1 activity

癌症研究 生物 流式细胞术 免疫印迹 癌症 甲基转移酶 细胞凋亡 细胞周期 车站3 癌细胞 癌基因 分子生物学 甲基化 基因 生物化学 遗传学
作者
Xuan Feng,Feng Xue,Guihua He,Qing Ni,Suiping Huang
出处
期刊:International Journal of Molecular Medicine [Spandidos Publishing]
卷期号:48 (2) 被引量:19
标识
DOI:10.3892/ijmm.2021.4998
摘要

Banxia xiexin decoction (BXXX) is a classic preparation used to treat gastrointestinal diseases, and also has certain therapeutic effects on gastrointestinal tumors. BXXX has been reported to regulate the expression of proteins associated with drug resistance and sensitivity in tumors, and thus, the aim of the present study was to investigate the mechanisms of BXXX drug sensitivity in gastric cancer (GC). The expression levels of programmed cell death 1 ligand 1 (PD‑L1), 6‑O‑methylguanine‑DNA methyltransferase (MGMT) and STAT3 were immunohistochemically detected in the cancer and adjacent non‑cancer tissues of patients with GC, and in vitro experimentation was conducted using drug‑resistant and ‑sensitive GC cells. The expression levels of PD‑L1, MGMT and STAT3 were determined using reverse transcription‑quantitative PCR. Different concentrations of BXXX drug serum were used to treat the cells and the cellular inhibition rate was assessed using a Cell Counting Kit‑8 assay. Flow cytometry was used to detect apoptosis, and western blot analysis was used to detect the expression levels of IL‑6, IFN‑γ, JAK/STAT3 pathway proteins, PD‑L1 and MGMT. The association between PD‑L1 and MGMT protein expression levels was subsequently assessed via co‑immunoprecipitation. Furthermore, in vivo studies were conducted following the establishment of a drug‑resistant tumor‑bearing mouse model, where GC tumor size was assessed under different treatment conditions, and western blot analysis was used to detect the expression of related pathway proteins. The expression levels of PD‑L1, MGMT and STAT3 were significantly increased in GC tissues, GC cells and cisplatin‑resistant cells. Furthermore, BXXX inhibited the proliferation of drug‑resistant cells and promoted the inhibitory effects of chemotherapeutic drugs on drug‑resistant cells. BXXX also inhibited the expression levels of IL‑6, IFN‑γ and JAK/STAT3 pathway proteins, as well as the expression levels of PD‑L1 and MGMT. Colivelin, an activator of STAT3, reversed the effects of BXXX on drug‑resistant GC cells, and significantly reversed the effect of BXXX on PD‑L1 expression. In conclusion, BXXX was found to influence the drug sensitivity of GC cells by regulating the expression of MGMT. This process functions viaPD‑L1, which was itself mediated by IL‑6/JAK/STAT3 signaling.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
PDF的下载单位、IP信息已删除 (2025-6-4)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
4秒前
邓权发布了新的文献求助10
10秒前
娇气的幼南完成签到 ,获得积分10
23秒前
25秒前
31秒前
47秒前
53秒前
生动之云发布了新的文献求助10
56秒前
1分钟前
1分钟前
美好颜发布了新的文献求助10
1分钟前
1分钟前
大模型应助科研通管家采纳,获得10
1分钟前
小二郎应助科研通管家采纳,获得10
1分钟前
Betty发布了新的文献求助10
2分钟前
2分钟前
2分钟前
2分钟前
2分钟前
慕青应助lty采纳,获得10
3分钟前
3分钟前
3分钟前
lty发布了新的文献求助10
3分钟前
小岩完成签到 ,获得积分10
3分钟前
3分钟前
咕咕发布了新的文献求助10
3分钟前
彩色黑米完成签到 ,获得积分10
3分钟前
科研通AI2S应助科研通管家采纳,获得10
3分钟前
4分钟前
4分钟前
AKi233完成签到,获得积分10
4分钟前
AKi233发布了新的文献求助10
4分钟前
充电宝应助AKi233采纳,获得10
4分钟前
咕咕完成签到,获得积分10
4分钟前
FengyaoWang完成签到,获得积分10
4分钟前
5分钟前
传奇3应助科研通管家采纳,获得10
5分钟前
英俊的铭应助科研通管家采纳,获得10
5分钟前
6分钟前
科研通AI5应助Betty采纳,获得10
6分钟前
高分求助中
A new approach to the extrapolation of accelerated life test data 1000
Cognitive Neuroscience: The Biology of the Mind 1000
Technical Brochure TB 814: LPIT applications in HV gas insulated switchgear 1000
Immigrant Incorporation in East Asian Democracies 600
Nucleophilic substitution in azasydnone-modified dinitroanisoles 500
不知道标题是什么 500
A Preliminary Study on Correlation Between Independent Components of Facial Thermal Images and Subjective Assessment of Chronic Stress 500
热门求助领域 (近24小时)
化学 材料科学 医学 生物 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 遗传学 基因 物理化学 催化作用 冶金 细胞生物学 免疫学
热门帖子
关注 科研通微信公众号,转发送积分 3968504
求助须知:如何正确求助?哪些是违规求助? 3513278
关于积分的说明 11167234
捐赠科研通 3248660
什么是DOI,文献DOI怎么找? 1794386
邀请新用户注册赠送积分活动 875030
科研通“疑难数据库(出版商)”最低求助积分说明 804638