转录后调控
蛋白质生物合成
分子生物学
核糖体RNA
抄写(语言学)
RNA结合蛋白
生物发生
多形体
核糖体蛋白
作者
Alison Galloway,Aneesa Kaskar,Dimitrinka Ditsova,Abdelmadjid Atrih,Harunori Yoshikawa,Carolina Gómez-Moreira,Olga Suska,Marcin Warmiński,Renata Grzela,Angus I. Lamond,Edward Darżynkiewicz,Jacek Jemielity,Victoria H. Cowling
摘要
The m7G cap is ubiquitous on RNAPII-transcribed RNA and has fundamental roles in eukaryotic gene expression, however its in vivo role in mammals has remained unknown. Here, we identified the m7G cap methyltransferase, RNMT, as a key mediator of T cell activation, which specifically regulates ribosome production. During T cell activation, induction of mRNA expression and ribosome biogenesis drives metabolic reprogramming, rapid proliferation and differentiation generating effector populations. We report that RNMT is induced by T cell receptor (TCR) stimulation and co-ordinates the mRNA, snoRNA and rRNA production required for ribosome biogenesis. Using transcriptomic and proteomic analyses, we demonstrate that RNMT selectively regulates the expression of terminal polypyrimidine tract (TOP) mRNAs, targets of the m7G-cap binding protein LARP1. The expression of LARP1 targets and snoRNAs involved in ribosome biogenesis is selectively compromised in Rnmt cKO CD4 T cells resulting in decreased ribosome synthesis, reduced translation rates and proliferation failure. By enhancing ribosome abundance, upregulation of RNMT co-ordinates mRNA capping and processing with increased translational capacity during T cell activation.
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