代谢工程
代谢途径
糖酵解
生物化学
辅因子
细菌
化学
合成生物学
功能(生物学)
乙酰辅酶A
生化工程
计算生物学
生物技术
生物
新陈代谢
酶
细胞生物学
遗传学
工程类
作者
Li Zhu,Jieze Zhang,Jiawei Yang,Yu Jiang,Sheng Yang
标识
DOI:10.1016/j.tibtech.2021.04.004
摘要
Acetyl CoA is an important precursor for various chemicals. We provide a metabolic engineering guideline for the production of acetyl-CoA and other end products from a bacterial chassis. Among 13 pathways that produce acetyl-CoA from glucose, 11 lose carbon in the process, and two do not. The first 11 use the Embden-Meyerhof-Parnas (EMP) pathway to produce redox cofactors and gain or lose ATP. The other two pathways function via phosphoketolase with net consumption of ATP, so they must therefore be combined with one of the 11 glycolytic pathways or auxiliary pathways. Optimization of these pathways can maximize the theoretical acetyl-CoA yield, thereby minimizing the overall cost of subsequent acetyl-CoA-derived molecules. Other strategies for generating hyper-producer strains are also addressed.
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