[RNA Binding Protein RBM47 Inhibits the K562 Cell Proliferation by Regulating HMGA2 mRNA Expression].

To investigate the regulatory effects of RBM47 on HMGA2 and the function of RBM47 in human chronic myeloid leukemia cell K562.K562 cells were transduction by the overexpressed and knockdown RBM47 lentiviral vector. CCK-8 assay was used to detect the effect of RBM47 on the proliferation of K562 cells. Flow cytometry assay was used to detect the effect of RBM47 on the cell cycle progression of K562 cells. RNA immunoprecipitation assay was used to detect the association between RBM47 and HMGA2 mRNA. RT-qPCR was used to detect the effects of RBM47 on the stability of HMGA2 mRNA. Western blot was used to evaluate the effect of RBM47 on HMGA2 protein expression.The overexpressed RBM47 could inhibit the proliferation and cell cycle progression of K562 cells. However, the inhibitation of RBM47 could improve the proliferation and cell cycle progression of K562 cells. RBM47 combined with HMGA2 mRNA could promote the degradation of HMGA2 mRNA. Thus, the overexpressed RBM47 could decrease the expression of HMGA2 protein in K562 cells.RNA binding protein RBM47 can inhibit the proliferation of K562 cells by regulating HMGA2 expression.RNA结合蛋白RBM47通过调节HMGA2抑制K562细胞的增殖.研究RBM47对下游基因HMGA2的调控作用，以及对人慢性髓系白血病细胞株K562增殖的调控作用.使用过表达、敲低RBM47的慢病毒载体感染K562细胞，CCK-8法检测RBM47对K562细胞增殖的影响，流式细胞术检测RBM47对K562细胞周期的影响；通过RNA免疫共沉淀结合方法检测RBM47与HMGA2 mRNA的结合情况，逆转录实时定量PCR方法检测RBM47对HMGA2 mRNA稳定性的影响，Western blot检测RBM47对HMGA2蛋白表达的影响.在K562细胞内过表达RBM47可抑制K562细胞的增殖和周期运行，抑制RBM47则可显著促进K562细胞增殖和细胞周期运行；RBM47可通过与HMGA2 mRNA结合促进其降解；过表达RBM47可显著降低K562细胞内HMGA2蛋白的表达.RNA结合蛋白RBM47通过调控HMGA2抑制K562细胞的增殖.