氧化应激
细胞凋亡
阿佩林
细胞色素c
化学
活性氧
神经毒性
药理学
SH-SY5Y型
半胱氨酸蛋白酶3
线粒体
钙
毒性
内分泌学
内科学
神经母细胞瘤
细胞培养
医学
生物化学
程序性细胞死亡
生物
受体
有机化学
遗传学
作者
Mohammad Rasoul Samandari-Bahraseman,Leila Elyasi
出处
期刊:Journal of basic and clinical physiology and pharmacology
[De Gruyter]
日期:2021-05-10
卷期号:33 (5): 599-605
被引量:14
标识
DOI:10.1515/jbcpp-2020-0294
摘要
We investigated the effect of apelin-13 on the cellular model of AD, amyloid-β (Aβ) treated SH-SY5Y cells in rats.The SH-SY5Y cells were pretreated with different doses of apelin-13 (1, 2.5, 5, and 10 μg/mL), half an hour before adding 50% Aβ treatment. After 24 h, cells were evaluated for survival, oxidative stress, mitochondrial calcium release, caspase-3, and cytochrome c levels, compared to control group (beta-actin). Statistical analysis was performed by SPSS 16.Apelin-13 at the dose of 2.5 μg/mL protected against IC50 Aβ (p<0.001). Apelin-13 at doses of 1, 2.5, and 5 μg/mL showed protective effects against the reactive oxygen species (ROS) produced by Aβ (p<0.001). Apelin-13 at doses of 2.5 and 5 μg/mL reduced calcium release, caspase-3, and cytochrome c (all p<0.001).Apelin-13 prevented apoptosis, oxidative stress, and mitochondrial toxicity and can be a suitable option for treatment of AD. The appropriate treatment strategy for humans has to be investigated in future studies.
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