Profiling Analysis of the Seeds of Vaccaria segetalis (Necr.) Gracke by HPLC-ESI-MS

In this paper, HPLC-ESI-MS technique was used to analyze the methanol extract of the seed of Vaccaria segetalis (Necr.) Gracke, known as Wang-Bu-Liu-Xing in traditional Chinese medicine. By the studies of MS and MS/MS spectra and the comparison with reference compounds, five main constituents were identified, including hypaphorine, vaccarin, isosaporarin, isovitexin 2''-O-arabinoside and vaccarin H.