凝胶渗透色谱法
校准曲线
色谱法
摩尔质量分布
化学
木质素
校准
超离心机
分子质量
渗透
扩散
分析化学(期刊)
聚合物
有机化学
膜
热力学
数学
物理
生物化学
统计
检出限
酶
作者
T. I. Obiaga,Morris Wayman
标识
DOI:10.1002/app.1974.070180704
摘要
In studying macromolecular properties of lignins, gel permeation chromatography (GPC) is a convenient method for the determination of lignin molecular weights and their distribution. A GPC analytical column was calibrated by preparing narrow molecular weight lignin fractions, then applying the method of Goring and co-workers to obtain molecular weights from the ultracentrifuge at zero concentration and zero field force. Diffusion and other curve-broadening effects in the GPC analytical column were taken into account by applying the correction method of Hamielec and co-workers to derive the coefficients of the calibration curve. The molecular weight of a lignosulfonate sample determined on the GPC was found to coincide closely with that obtained by ultracentrifugation.
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