蜕皮激素
菠菜
20-羟基蜕皮激素
菠菜
化学
相(物质)
色谱法
水溶液
红头丽蝇
环氧乙烷
聚合物
有机化学
植物
生物化学
共聚物
激素
生物
叶绿体
幼虫
丽蝇科
基因
作者
Richard F. Modlin,Patricia Alred,Folke Tjerneld
标识
DOI:10.1016/0021-9673(94)80112-6
摘要
An aqueous solution of the ethylene oxide-propylene oxide random copolymer UCON 50-HB-5100 was successfully used to extract ecdysone and 20-hydroxyecdysone from the common spinach plant, Spinacia oleracea. The UCON spinach extract was mixed with a hydroxypropyl starch Reppal PES 200 solution and allowed to form an aqueous two-phase system. After the polymers separated cell debris, proteins and other comtaminants partitioned to the lower Reppal phase and ecdysone and 20-hydroxyecdysone partitioned to the upper UCON phase. The UCON phase was isolated and subjected to a temperature increase to 56°C which induced phase separation between UCON and water. Ecdysone and 20-hydroxyecdysone partitioned between the UCON phase and the water phase at concentrations determined by their degree of hydrophobicity. The less hydrophobic 20-hydroxyecdysone had a greater affinity for the water-rich phase than did ecdysone. Due to the larger volume of the water phase both ecdysteroids were obtained in this phase at 56°C with yields higher than 80%. With 20% ethanol in the primary system recovery was 88.7% for ecdysone and 91.2% for 20-hydroxyecdysone. Results indicate that aqueous two-phase partitioning coupled with temperature-induced phase separation is a quick, easy and inexpensive bench-top technique for extracting and purifying ecdysteroids from raw material. This technique can also be readily up-scaled for commercial use.
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