Compounds from the Chinese black ant (Polyrhachis dives) and NMR behavior of the isomers with formamide group

AbstractTwo new dopamine derivatives divesamides A (1) and B (2), along with six known N-containing compounds were isolated from the Chinese black ant (Polyrhachis dives). Their structures were determined on the basis of spectroscopic methods. Compound 1 is a racemate, and chiral HPLC separation yielded a pair of antipodes. The absolute configuration of (+)-1 was assigned by a computational method. The double signals in the 1H and 13C NMR spectra of 2 that resulted from the presence of a formamide group were discussed. The T- and B-lymphocytes proliferation assay showed that 2 has moderate immunosuppressive activity toward T- and B-lymphocytes proliferation at a concentration of 20 μM.Keywords:: Polyrhachis divesblack antN-containing compoundamide bondimmunosuppressive activity AcknowledgmentsThis work was financially supported by the Open Research Fund of State Key Laboratory Breeding Base of Systematic Research, Development and Utilization of Chinese Medicine Resources.Notes1. These authors contributed equally to this paper.