Transcriptome, enzyme activity and histopathology analysis reveal the effects of dietary carbohydrate on glycometabolism in juvenile largemouth bass, Micropterus salmoides

生物 微翅目 小桶 鲈鱼(鱼) 转录组 碳水化合物代谢 基因 基因表达谱 遗传学 生物化学 基因表达 生态学
作者
Wei Zhang,Kang Liu,Hongyu Liu,Xiaohui Dong,Shuyan Chi,Shuang Zhang,Hualang Wang
出处
期刊:Aquaculture [Elsevier BV]
卷期号:504: 39-51 被引量:39
标识
DOI:10.1016/j.aquaculture.2019.01.030
摘要

Largemouth bass (Micropterus salmoides) is one of the most valuable freshwater aquatic species in China. However, M. salmoides appeared to use dietary carbohydrate more poorly than other carnivorous fish. Although carbohydrate metabolism of fish has been well studied for the past decades, studies regarding transcriptomic profiling of carbohydrate metabolism in fish are lacking. In this study, the transcriptomic profiles of M. salmoides liver were explored for the first time by feeding seven kinds of diets, and selectively analyzed the glycometabolism at different levels of dietary carbohydrate (ML-120 g kg−1, MM-240 g kg−1, and MH-360 g kg−1). Totally, the de novo assemblies yielded 50,743 unigenes with an average of 1169 bp. The assembled unigenes were evaluated and functionally annotated by comparing with sequences in major public databases including protein sequence (Nr), Clusters of Orthologous Groups (COG), Swiss-Prot, Kyoto Encyclopedia of Genes and Genomes (KEGG) and Gene Ontology database (GO). Comparison of gene expression levels revealed that differentially expressed genes (DEGs) (P < .05): a total of 9096 (613 up-regulated and 8483 down-regulated genes) was obtained between ML and MM; 5778 (414 up-regulated and 5365 down-regulated) was obtained between MH and MM. Moreover, 12 carbohydrate metabolism-related genes were selected for quantitative real-time PCR analysis to validate the RNA-Seq data. The results confirmed the consistency of the expression levels between RNA-Seq and qRT-PCR data. Meanwhile, present study also examined the related enzyme activities of carbohydrate metabolism. The results showed that high level of dietary carbohydrate (360 g kg−1) exhibited a strong inhibitory effect on the genes of M. salmoides. Dietary carbohydrate in MM up-regulated 93.3% of differential expression genes (DEGs) compared to ML, however, 92.9% of the DEGs in MH were depressed compared to MM. This trend is also reflected in the corresponding enzyme activities in general. With dietary carbohydrate increase, there were severe histological alterations in M. salmoides liver. Collectively, this study shows a systematic overview of the transcriptome analysis in M. salmoides, and provides valuable gene information for studying molecular mechanisms of carbohydrate metabolism in freshwater fish.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
PDF的下载单位、IP信息已删除 (2025-6-4)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
末末完成签到 ,获得积分10
1秒前
无为完成签到 ,获得积分10
2秒前
白嫖论文完成签到 ,获得积分10
4秒前
上官若男应助忧伤的步美采纳,获得10
7秒前
科研通AI2S应助科研通管家采纳,获得10
7秒前
10秒前
从心随缘完成签到 ,获得积分10
11秒前
花花发布了新的文献求助10
13秒前
牛奶面包完成签到 ,获得积分10
14秒前
15秒前
岁月如歌完成签到 ,获得积分0
15秒前
18秒前
Li完成签到,获得积分10
20秒前
张琨完成签到 ,获得积分10
20秒前
20秒前
sunnyqqz完成签到,获得积分10
23秒前
热情的乘风完成签到,获得积分20
23秒前
25秒前
霍凡白完成签到,获得积分10
26秒前
27秒前
Feng发布了新的文献求助20
28秒前
怕孤单的若颜完成签到 ,获得积分10
30秒前
31秒前
ruochenzu发布了新的文献求助10
34秒前
zhongu发布了新的文献求助10
38秒前
阳光彩虹小白马完成签到 ,获得积分10
38秒前
Feng完成签到,获得积分10
40秒前
花花完成签到,获得积分10
42秒前
45秒前
量子星尘发布了新的文献求助10
47秒前
杨一完成签到 ,获得积分10
50秒前
猫猫头完成签到 ,获得积分10
51秒前
53秒前
56秒前
忒寒碜完成签到,获得积分10
1分钟前
1分钟前
XU博士完成签到,获得积分10
1分钟前
哭泣青烟完成签到 ,获得积分10
1分钟前
roundtree完成签到 ,获得积分0
1分钟前
等待谷南完成签到,获得积分10
1分钟前
高分求助中
【提示信息,请勿应助】关于scihub 10000
Les Mantodea de Guyane: Insecta, Polyneoptera [The Mantids of French Guiana] 3000
徐淮辽南地区新元古代叠层石及生物地层 3000
The Mother of All Tableaux: Order, Equivalence, and Geometry in the Large-scale Structure of Optimality Theory 3000
Handbook of Industrial Diamonds.Vol2 1100
Global Eyelash Assessment scale (GEA) 1000
Picture Books with Same-sex Parented Families: Unintentional Censorship 550
热门求助领域 (近24小时)
化学 材料科学 医学 生物 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 遗传学 基因 物理化学 催化作用 冶金 细胞生物学 免疫学
热门帖子
关注 科研通微信公众号,转发送积分 4038039
求助须知:如何正确求助?哪些是违规求助? 3575756
关于积分的说明 11373782
捐赠科研通 3305574
什么是DOI,文献DOI怎么找? 1819239
邀请新用户注册赠送积分活动 892655
科研通“疑难数据库(出版商)”最低求助积分说明 815022