白色念珠菌
酵母
表面电荷
纳米颗粒
生物物理学
静电
细胞壁
白色体
荧光显微镜
材料科学
化学
纳米技术
荧光
生物
微生物学
生物化学
物理
物理化学
量子力学
作者
Tian Wang,Fan Li,Sheng‐Ming Wu,Gen Li,Lieying Fan,Xue Qu,Xin‐Ming Jia,Yilong Wang
出处
期刊:ACS Biomaterials Science & Engineering
[American Chemical Society]
日期:2019-06-03
卷期号:5 (7): 3270-3278
被引量:5
标识
DOI:10.1021/acsbiomaterials.9b00069
摘要
The early detection of fungi through a facile and straightforward method is desirable. The isolation of fungi from a body fluid sample plays a major role in effective detection. In the past, concanavalinA (conA), one of the lectins, interacted with Candida albicans through the surface component of the yeast cell wall. The development of a facile method with a robust binding affinity and an efficient capture of the yeast in addition to conA could pose a potential for the sandwich-like assay of C. albicans. In this study, the feasibility of an electrostatic interaction-mediated yeast capture was investigated as compared to conA-mediated binding. Also, the optimal parameters for the efficient isolation of C. albicans by surface-charged nanoparticles were studied, and the mechanism of the binding site through the electrostatic interaction on the surface of the yeast cell wall was explored by a blocking experiment. Furthermore, the yeast strains were found to interact uniformly only via the positively charged nanoparticles, and the captured yeast could be analyzed by FITC-conA fluorescence staining and T2 magnetic resonance assay. Thus, this strategy established a rapid and highly efficient method for the isolation of fungi and analysis.
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