An outbreak of multiply antibiotic-resistant ST49:ST128:KL11:OCL8 Acinetobacter baumannii isolates at a Sydney hospital

微生物学 爆发 抗生素 医学 脉冲场凝胶电泳 不动杆菌 抗药性 粘菌素 多重耐药 庆大霉素 美罗培南 阿米卡星 亚胺培南 舒巴坦钠 替加环素 抗菌剂 环丙沙星 氨苄西林 多位点序列分型
作者
Mohammad Hamidian,Stephanie J Ambrose,Grace A. Blackwell,Steven J. Nigro,Ruth M. Hall
出处
期刊:Journal of Antimicrobial Chemotherapy [Oxford University Press]
卷期号:76 (4): 893-900 被引量:3
标识
DOI:10.1093/jac/dkaa553
摘要

Objectives To understand the acquisition of resistance genes by a non-GC1, non-GC2 Acinetobacter baumannii strain responsible for a 4 year outbreak at a Sydney hospital. Methods Representative isolates were screened for resistance to antibiotics. Three were subjected to WGS using Illumina HiSeq. One genome was completed with MinION long reads. Resistance regions were compared with known sequences using bioinformatics. Results Isolates were resistant to third-generation cephalosporins, gentamicin and tobramycin, sulfamethoxazole and erythromycin. Sequenced isolates were ST49 (Institut Pasteur scheme) and ST128 (Oxford scheme) and carried KL11 at the capsule locus and OCL8 at the lipooligosaccharide outer core locus. The complete genome of isolate J9 revealed that the resistance genes were all in plasmids; pRAY* contained aadB, and a large plasmid, pJ9-3, contained sul2 and floR genes and a dif module containing the mph(E)-msr(E) macrolide resistance genes. Transposon Tn6168, consisting of a second copy of the chromosomal ampC gene region flanked by ISAba1s, confers resistance to third-generation cephalosporins. Tn6168 is located inside the mph(E)-msr(E) dif module. pJ9-3 includes a set of four dif modules and the orientation of the pdif sites, XerC-XerD or XerD-XerC, alternates. A large transposon, Tn6175, containing tniCABDE transposition genes and genes annotated as being involved in heavy metal metabolism, uptake or export was found in the comM gene. Other ST49:ST128:KL11:OCL8 genomes found in the GenBank WGS database carried Tn6175 but neither of the plasmids carrying the resistance genes. Conclusions An early carbapenem-susceptible A. baumannii outbreak recorded in Australia was caused by an unusual clone that had acquired plasmids carrying antibiotic resistance genes.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
大幅提高文件上传限制,最高150M (2024-4-1)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
单薄不惜完成签到,获得积分10
2秒前
感动的小鸭子完成签到 ,获得积分10
3秒前
zqw完成签到,获得积分10
4秒前
4秒前
chen完成签到,获得积分10
4秒前
丁凛完成签到,获得积分10
4秒前
aoao嘉完成签到,获得积分10
4秒前
cy关注了科研通微信公众号
5秒前
Ininn完成签到 ,获得积分10
5秒前
lanmin完成签到,获得积分20
6秒前
耍酷的翠曼完成签到,获得积分10
6秒前
人参跳芭蕾完成签到 ,获得积分10
6秒前
慕容冷之完成签到,获得积分10
6秒前
微笑的井完成签到 ,获得积分10
7秒前
qingxinhuo完成签到 ,获得积分10
7秒前
11完成签到,获得积分10
7秒前
7秒前
zhangyidian完成签到,获得积分10
8秒前
胡萝卜发布了新的文献求助10
8秒前
2041完成签到,获得积分10
8秒前
舒洛完成签到,获得积分10
8秒前
9秒前
优雅莞完成签到,获得积分10
9秒前
Rebekah完成签到,获得积分0
9秒前
斯文败类应助zhvjdb采纳,获得10
10秒前
香蕉觅云应助派大星采纳,获得10
11秒前
苯二氮卓完成签到 ,获得积分10
11秒前
12秒前
江边鸟完成签到 ,获得积分10
12秒前
阳光下的味道完成签到,获得积分10
12秒前
eno完成签到,获得积分10
12秒前
lw完成签到,获得积分10
12秒前
小九发布了新的文献求助10
12秒前
xxp发布了新的文献求助10
13秒前
正经大善人完成签到,获得积分10
13秒前
13秒前
Gilana应助xiaoxin采纳,获得80
13秒前
paulmichael发布了新的文献求助10
14秒前
阿千完成签到 ,获得积分10
14秒前
123完成签到,获得积分10
15秒前
高分求助中
Spray / Wall-interaction Modelling by Dimensionless Data Analysis 2000
Write Like a Chemist: A Guide and Resource (第二版) 600
Mixed-anion Compounds 600
A new species of Velataspis (Hemiptera Coccoidea Diaspididae) from tea in Assam 500
Earth System Geophysics 500
Aspects of Babylonian celestial divination: the lunar eclipse tablets of Enūma Anu Enlil 500
Geochemistry, 2nd Edition 地球化学经典教科书第二版 401
热门求助领域 (近24小时)
化学 医学 生物 材料科学 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 基因 遗传学 催化作用 物理化学 免疫学 量子力学 细胞生物学
热门帖子
关注 科研通微信公众号,转发送积分 3200846
求助须知:如何正确求助?哪些是违规求助? 2850652
关于积分的说明 8073045
捐赠科研通 2514396
什么是DOI,文献DOI怎么找? 1347144
科研通“疑难数据库(出版商)”最低求助积分说明 640332
邀请新用户注册赠送积分活动 610525