亲爱的研友该休息了!由于当前在线用户较少,发布求助请尽量完整的填写文献信息,科研通机器人24小时在线,伴您度过漫漫科研夜!身体可是革命的本钱,早点休息,好梦!

An outbreak of multiply antibiotic-resistant ST49:ST128:KL11:OCL8 Acinetobacter baumannii isolates at a Sydney hospital

微生物学 爆发 抗生素 医学 脉冲场凝胶电泳 不动杆菌 抗药性 粘菌素 多重耐药 庆大霉素 美罗培南 阿米卡星 亚胺培南 舒巴坦钠 替加环素 抗菌剂 环丙沙星 氨苄西林 多位点序列分型
作者
Mohammad Hamidian,Stephanie J Ambrose,Grace A. Blackwell,Steven J. Nigro,Ruth M. Hall
出处
期刊:Journal of Antimicrobial Chemotherapy [Oxford University Press]
卷期号:76 (4): 893-900 被引量:3
标识
DOI:10.1093/jac/dkaa553
摘要

Objectives To understand the acquisition of resistance genes by a non-GC1, non-GC2 Acinetobacter baumannii strain responsible for a 4 year outbreak at a Sydney hospital. Methods Representative isolates were screened for resistance to antibiotics. Three were subjected to WGS using Illumina HiSeq. One genome was completed with MinION long reads. Resistance regions were compared with known sequences using bioinformatics. Results Isolates were resistant to third-generation cephalosporins, gentamicin and tobramycin, sulfamethoxazole and erythromycin. Sequenced isolates were ST49 (Institut Pasteur scheme) and ST128 (Oxford scheme) and carried KL11 at the capsule locus and OCL8 at the lipooligosaccharide outer core locus. The complete genome of isolate J9 revealed that the resistance genes were all in plasmids; pRAY* contained aadB, and a large plasmid, pJ9-3, contained sul2 and floR genes and a dif module containing the mph(E)-msr(E) macrolide resistance genes. Transposon Tn6168, consisting of a second copy of the chromosomal ampC gene region flanked by ISAba1s, confers resistance to third-generation cephalosporins. Tn6168 is located inside the mph(E)-msr(E) dif module. pJ9-3 includes a set of four dif modules and the orientation of the pdif sites, XerC-XerD or XerD-XerC, alternates. A large transposon, Tn6175, containing tniCABDE transposition genes and genes annotated as being involved in heavy metal metabolism, uptake or export was found in the comM gene. Other ST49:ST128:KL11:OCL8 genomes found in the GenBank WGS database carried Tn6175 but neither of the plasmids carrying the resistance genes. Conclusions An early carbapenem-susceptible A. baumannii outbreak recorded in Australia was caused by an unusual clone that had acquired plasmids carrying antibiotic resistance genes.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
大幅提高文件上传限制,最高150M (2024-4-1)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
ganggangfu完成签到,获得积分0
8秒前
年轻千愁完成签到 ,获得积分10
9秒前
ganggang完成签到,获得积分0
18秒前
41秒前
呆呆兽发布了新的文献求助10
1分钟前
丘比特应助科研通管家采纳,获得10
2分钟前
CodeCraft应助烽烽烽采纳,获得10
3分钟前
香蕉以菱完成签到 ,获得积分10
3分钟前
领导范儿应助呆呆兽采纳,获得10
4分钟前
4分钟前
HAHA磾发布了新的文献求助10
4分钟前
HAHA磾完成签到,获得积分10
4分钟前
熊熊完成签到,获得积分10
4分钟前
Georgechan完成签到,获得积分10
4分钟前
5分钟前
呆呆兽发布了新的文献求助10
5分钟前
NexusExplorer应助JazzWon采纳,获得10
6分钟前
研友_VZG7GZ应助科研通管家采纳,获得10
6分钟前
从心随缘完成签到 ,获得积分10
6分钟前
呆呆兽发布了新的文献求助30
7分钟前
兴尽晚回舟完成签到 ,获得积分10
7分钟前
8分钟前
JazzWon发布了新的文献求助10
8分钟前
8分钟前
随机子应助科研通管家采纳,获得10
8分钟前
9分钟前
激情的蜗牛发布了新的文献求助200
9分钟前
呆呆兽发布了新的文献求助10
9分钟前
共享精神应助呆呆兽采纳,获得10
9分钟前
9分钟前
sq发布了新的文献求助10
10分钟前
ling361完成签到,获得积分10
10分钟前
JazzWon完成签到,获得积分10
10分钟前
h0jian09完成签到,获得积分10
10分钟前
11分钟前
李健的小迷弟应助孙亦沈采纳,获得10
11分钟前
呆呆兽发布了新的文献求助10
11分钟前
Laiiiiieng发布了新的文献求助10
12分钟前
王子语完成签到,获得积分10
13分钟前
Jayden完成签到 ,获得积分10
13分钟前
高分求助中
Spray / Wall-interaction Modelling by Dimensionless Data Analysis 2000
Mathematics and Finite Element Discretizations of Incompressible Navier—Stokes Flows 500
A new species of Coccus (Homoptera: Coccoidea) from Malawi 500
2-Acetyl-1-pyrroline: an important aroma component of cooked rice 500
A real-time energy management strategy based on fuzzy control and ECMS for PHEVs 400
Handbook on People's China (1957) 400
2024 Medicinal Chemistry Reviews 400
热门求助领域 (近24小时)
化学 医学 生物 材料科学 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 基因 遗传学 催化作用 物理化学 免疫学 量子力学 细胞生物学
热门帖子
关注 科研通微信公众号,转发送积分 3190002
求助须知:如何正确求助?哪些是违规求助? 2839275
关于积分的说明 8023343
捐赠科研通 2502162
什么是DOI,文献DOI怎么找? 1336333
科研通“疑难数据库(出版商)”最低求助积分说明 637818
邀请新用户注册赠送积分活动 605932