An outbreak of multiply antibiotic-resistant ST49:ST128:KL11:OCL8 Acinetobacter baumannii isolates at a Sydney hospital

微生物学 爆发 抗生素 医学 脉冲场凝胶电泳 不动杆菌 抗药性 粘菌素 多重耐药 庆大霉素 美罗培南 阿米卡星 亚胺培南 舒巴坦钠 替加环素 抗菌剂 环丙沙星 氨苄西林 多位点序列分型
作者
Mohammad Hamidian,Stephanie J Ambrose,Grace A. Blackwell,Steven J. Nigro,Ruth M. Hall
出处
期刊:Journal of Antimicrobial Chemotherapy [Oxford University Press]
卷期号:76 (4): 893-900 被引量:3
标识
DOI:10.1093/jac/dkaa553
摘要

Objectives To understand the acquisition of resistance genes by a non-GC1, non-GC2 Acinetobacter baumannii strain responsible for a 4 year outbreak at a Sydney hospital. Methods Representative isolates were screened for resistance to antibiotics. Three were subjected to WGS using Illumina HiSeq. One genome was completed with MinION long reads. Resistance regions were compared with known sequences using bioinformatics. Results Isolates were resistant to third-generation cephalosporins, gentamicin and tobramycin, sulfamethoxazole and erythromycin. Sequenced isolates were ST49 (Institut Pasteur scheme) and ST128 (Oxford scheme) and carried KL11 at the capsule locus and OCL8 at the lipooligosaccharide outer core locus. The complete genome of isolate J9 revealed that the resistance genes were all in plasmids; pRAY* contained aadB, and a large plasmid, pJ9-3, contained sul2 and floR genes and a dif module containing the mph(E)-msr(E) macrolide resistance genes. Transposon Tn6168, consisting of a second copy of the chromosomal ampC gene region flanked by ISAba1s, confers resistance to third-generation cephalosporins. Tn6168 is located inside the mph(E)-msr(E) dif module. pJ9-3 includes a set of four dif modules and the orientation of the pdif sites, XerC-XerD or XerD-XerC, alternates. A large transposon, Tn6175, containing tniCABDE transposition genes and genes annotated as being involved in heavy metal metabolism, uptake or export was found in the comM gene. Other ST49:ST128:KL11:OCL8 genomes found in the GenBank WGS database carried Tn6175 but neither of the plasmids carrying the resistance genes. Conclusions An early carbapenem-susceptible A. baumannii outbreak recorded in Australia was caused by an unusual clone that had acquired plasmids carrying antibiotic resistance genes.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
大幅提高文件上传限制,最高150M (2024-4-1)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
clock发布了新的文献求助30
刚刚
091完成签到 ,获得积分10
刚刚
桐桐应助仗炮由纪采纳,获得10
1秒前
lb发布了新的文献求助10
1秒前
bjyx发布了新的文献求助10
1秒前
1秒前
苏卿应助taowang14采纳,获得10
2秒前
2秒前
卜青发布了新的文献求助10
2秒前
3秒前
3秒前
时光宝石一次完成签到,获得积分10
4秒前
和谐灯泡发布了新的文献求助10
4秒前
bkagyin应助碳TENET碳采纳,获得10
5秒前
6秒前
00小费0发布了新的文献求助10
6秒前
纯真冰露发布了新的文献求助10
6秒前
nancyes完成签到 ,获得积分10
7秒前
SciGPT应助南巷酒肆采纳,获得10
8秒前
8秒前
袁奇点发布了新的文献求助30
8秒前
坦率的香烟完成签到 ,获得积分10
9秒前
科研通AI2S应助冷傲机器猫采纳,获得10
9秒前
9秒前
9秒前
FashionBoy应助xf采纳,获得10
9秒前
研友_VZG7GZ应助bjyx采纳,获得10
11秒前
11秒前
jiejie发布了新的文献求助10
11秒前
吴大语完成签到,获得积分10
12秒前
12秒前
DJHKFD完成签到,获得积分10
13秒前
13秒前
lvbowen发布了新的文献求助20
15秒前
15秒前
卜青完成签到,获得积分10
15秒前
如意千万应助无奈曼云采纳,获得50
15秒前
sy完成签到,获得积分20
16秒前
17秒前
lb完成签到,获得积分20
17秒前
高分求助中
Evolution 3rd edition 1500
保险藏宝图 1000
Lire en communiste 1000
Mantiden: Faszinierende Lauerjäger Faszinierende Lauerjäger 700
PraxisRatgeber: Mantiden: Faszinierende Lauerjäger 700
A new species of Coccus (Homoptera: Coccoidea) from Malawi 500
2-Acetyl-1-pyrroline: an important aroma component of cooked rice 500
热门求助领域 (近24小时)
化学 医学 生物 材料科学 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 基因 遗传学 催化作用 物理化学 免疫学 量子力学 细胞生物学
热门帖子
关注 科研通微信公众号,转发送积分 3184197
求助须知:如何正确求助?哪些是违规求助? 2834382
关于积分的说明 7999641
捐赠科研通 2496672
什么是DOI,文献DOI怎么找? 1332496
科研通“疑难数据库(出版商)”最低求助积分说明 636594
邀请新用户注册赠送积分活动 603903