An outbreak of multiply antibiotic-resistant ST49:ST128:KL11:OCL8 Acinetobacter baumannii isolates at a Sydney hospital

微生物学 爆发 抗生素 医学 脉冲场凝胶电泳 不动杆菌 抗药性 粘菌素 多重耐药 庆大霉素 美罗培南 阿米卡星 亚胺培南 舒巴坦钠 替加环素 抗菌剂 环丙沙星 氨苄西林 多位点序列分型
作者
Mohammad Hamidian,Stephanie J Ambrose,Grace A. Blackwell,Steven J. Nigro,Ruth M. Hall
出处
期刊:Journal of Antimicrobial Chemotherapy [Oxford University Press]
卷期号:76 (4): 893-900 被引量:3
标识
DOI:10.1093/jac/dkaa553
摘要

Objectives To understand the acquisition of resistance genes by a non-GC1, non-GC2 Acinetobacter baumannii strain responsible for a 4 year outbreak at a Sydney hospital. Methods Representative isolates were screened for resistance to antibiotics. Three were subjected to WGS using Illumina HiSeq. One genome was completed with MinION long reads. Resistance regions were compared with known sequences using bioinformatics. Results Isolates were resistant to third-generation cephalosporins, gentamicin and tobramycin, sulfamethoxazole and erythromycin. Sequenced isolates were ST49 (Institut Pasteur scheme) and ST128 (Oxford scheme) and carried KL11 at the capsule locus and OCL8 at the lipooligosaccharide outer core locus. The complete genome of isolate J9 revealed that the resistance genes were all in plasmids; pRAY* contained aadB, and a large plasmid, pJ9-3, contained sul2 and floR genes and a dif module containing the mph(E)-msr(E) macrolide resistance genes. Transposon Tn6168, consisting of a second copy of the chromosomal ampC gene region flanked by ISAba1s, confers resistance to third-generation cephalosporins. Tn6168 is located inside the mph(E)-msr(E) dif module. pJ9-3 includes a set of four dif modules and the orientation of the pdif sites, XerC-XerD or XerD-XerC, alternates. A large transposon, Tn6175, containing tniCABDE transposition genes and genes annotated as being involved in heavy metal metabolism, uptake or export was found in the comM gene. Other ST49:ST128:KL11:OCL8 genomes found in the GenBank WGS database carried Tn6175 but neither of the plasmids carrying the resistance genes. Conclusions An early carbapenem-susceptible A. baumannii outbreak recorded in Australia was caused by an unusual clone that had acquired plasmids carrying antibiotic resistance genes.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
大幅提高文件上传限制,最高150M (2024-4-1)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
快乐惜灵给快乐惜灵的求助进行了留言
1秒前
ziyiziyi发布了新的文献求助10
1秒前
万信心完成签到 ,获得积分20
1秒前
Jabowoo完成签到,获得积分10
1秒前
1秒前
violet3zz应助凤凤采纳,获得10
1秒前
上官若男应助凤凤采纳,获得10
1秒前
典雅的青旋完成签到,获得积分20
1秒前
孤檠应助研友_Z33zkZ采纳,获得10
1秒前
小乔应助研友_Z33zkZ采纳,获得10
1秒前
2秒前
加贝发布了新的文献求助10
2秒前
moon发布了新的文献求助10
2秒前
2秒前
思源应助戈惜采纳,获得10
3秒前
zsdad发布了新的文献求助10
3秒前
4秒前
4秒前
无足鸟应助AI倩采纳,获得10
4秒前
你曾是少年完成签到,获得积分10
5秒前
HEIKU应助科研通管家采纳,获得10
5秒前
科研通AI2S应助科研通管家采纳,获得10
5秒前
烟花应助科研通管家采纳,获得10
5秒前
5秒前
毛豆应助科研通管家采纳,获得10
5秒前
Orange应助科研通管家采纳,获得10
5秒前
Orange应助科研通管家采纳,获得10
5秒前
Lucas应助科研通管家采纳,获得10
5秒前
充电宝应助科研通管家采纳,获得10
5秒前
毛豆应助科研通管家采纳,获得10
6秒前
大宇完成签到,获得积分10
6秒前
6秒前
HEIKU应助科研通管家采纳,获得10
6秒前
HEIKU应助科研通管家采纳,获得10
6秒前
不配.应助科研通管家采纳,获得10
6秒前
HEIKU应助科研通管家采纳,获得10
6秒前
传奇3应助科研通管家采纳,获得10
6秒前
iNk应助科研通管家采纳,获得10
6秒前
iNk应助科研通管家采纳,获得10
6秒前
烟花应助科研通管家采纳,获得30
6秒前
高分求助中
Spray / Wall-interaction Modelling by Dimensionless Data Analysis 2000
ALA生合成不全マウスでの糖代謝異常の分子機構解析 520
安全防范技术与工程 500
Mathematics and Finite Element Discretizations of Incompressible Navier—Stokes Flows 500
2024 Medicinal Chemistry Reviews 400
Актуализированная стратиграфическая схема триасовых отложений Прикаспийского региона. Объяснительная записка 360
Dictionary of socialism 350
热门求助领域 (近24小时)
化学 医学 生物 材料科学 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 基因 遗传学 催化作用 物理化学 免疫学 量子力学 细胞生物学
热门帖子
关注 科研通微信公众号,转发送积分 3193089
求助须知:如何正确求助?哪些是违规求助? 2842144
关于积分的说明 8037556
捐赠科研通 2505979
什么是DOI,文献DOI怎么找? 1338850
科研通“疑难数据库(出版商)”最低求助积分说明 638545
邀请新用户注册赠送积分活动 607073