亲爱的研友该休息了!由于当前在线用户较少,发布求助请尽量完整的填写文献信息,科研通机器人24小时在线,伴您度过漫漫科研夜!身体可是革命的本钱,早点休息,好梦!

An outbreak of multiply antibiotic-resistant ST49:ST128:KL11:OCL8 Acinetobacter baumannii isolates at a Sydney hospital

微生物学 爆发 抗生素 医学 脉冲场凝胶电泳 不动杆菌 抗药性 粘菌素 多重耐药 庆大霉素 美罗培南 阿米卡星 亚胺培南 舒巴坦钠 替加环素 抗菌剂 环丙沙星 氨苄西林 多位点序列分型
作者
Mohammad Hamidian,Stephanie J Ambrose,Grace A. Blackwell,Steven J. Nigro,Ruth M. Hall
出处
期刊:Journal of Antimicrobial Chemotherapy [Oxford University Press]
卷期号:76 (4): 893-900 被引量:3
标识
DOI:10.1093/jac/dkaa553
摘要

Objectives To understand the acquisition of resistance genes by a non-GC1, non-GC2 Acinetobacter baumannii strain responsible for a 4 year outbreak at a Sydney hospital. Methods Representative isolates were screened for resistance to antibiotics. Three were subjected to WGS using Illumina HiSeq. One genome was completed with MinION long reads. Resistance regions were compared with known sequences using bioinformatics. Results Isolates were resistant to third-generation cephalosporins, gentamicin and tobramycin, sulfamethoxazole and erythromycin. Sequenced isolates were ST49 (Institut Pasteur scheme) and ST128 (Oxford scheme) and carried KL11 at the capsule locus and OCL8 at the lipooligosaccharide outer core locus. The complete genome of isolate J9 revealed that the resistance genes were all in plasmids; pRAY* contained aadB, and a large plasmid, pJ9-3, contained sul2 and floR genes and a dif module containing the mph(E)-msr(E) macrolide resistance genes. Transposon Tn6168, consisting of a second copy of the chromosomal ampC gene region flanked by ISAba1s, confers resistance to third-generation cephalosporins. Tn6168 is located inside the mph(E)-msr(E) dif module. pJ9-3 includes a set of four dif modules and the orientation of the pdif sites, XerC-XerD or XerD-XerC, alternates. A large transposon, Tn6175, containing tniCABDE transposition genes and genes annotated as being involved in heavy metal metabolism, uptake or export was found in the comM gene. Other ST49:ST128:KL11:OCL8 genomes found in the GenBank WGS database carried Tn6175 but neither of the plasmids carrying the resistance genes. Conclusions An early carbapenem-susceptible A. baumannii outbreak recorded in Australia was caused by an unusual clone that had acquired plasmids carrying antibiotic resistance genes.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
大幅提高文件上传限制,最高150M (2024-4-1)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
1秒前
觅雪格发布了新的文献求助10
17秒前
华仔应助鱼日采纳,获得150
32秒前
38秒前
1分钟前
Charlie完成签到 ,获得积分10
1分钟前
2分钟前
觅雪格完成签到 ,获得积分10
2分钟前
通科研完成签到 ,获得积分10
3分钟前
3分钟前
3分钟前
3分钟前
CGBY完成签到 ,获得积分10
4分钟前
4分钟前
5分钟前
5分钟前
5分钟前
6分钟前
杨同学发布了新的文献求助10
6分钟前
6分钟前
甜蜜发带完成签到 ,获得积分10
6分钟前
yyr完成签到 ,获得积分20
6分钟前
6分钟前
7分钟前
葛力完成签到,获得积分10
7分钟前
7分钟前
7分钟前
执着的飞瑶完成签到,获得积分20
7分钟前
毛豆应助司徒无剑采纳,获得20
7分钟前
7分钟前
8分钟前
8分钟前
8分钟前
赘婿应助Sandy采纳,获得10
8分钟前
9分钟前
刻苦羽毛完成签到 ,获得积分10
9分钟前
9分钟前
10分钟前
10分钟前
10分钟前
高分求助中
Spray / Wall-interaction Modelling by Dimensionless Data Analysis 2000
Aspects of Babylonian celestial divination: the lunar eclipse tablets of Enūma Anu Enlil 500
Mathematics and Finite Element Discretizations of Incompressible Navier—Stokes Flows 500
Dictionary of socialism 350
Mixed-anion Compounds 300
Geochemistry, 2nd Edition 地球化学经典教科书第二版 300
Idoxuridine 260
热门求助领域 (近24小时)
化学 医学 生物 材料科学 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 基因 遗传学 催化作用 物理化学 免疫学 量子力学 细胞生物学
热门帖子
关注 科研通微信公众号,转发送积分 3196776
求助须知:如何正确求助?哪些是违规求助? 2845469
关于积分的说明 8054191
捐赠科研通 2510065
什么是DOI,文献DOI怎么找? 1342246
科研通“疑难数据库(出版商)”最低求助积分说明 639349
邀请新用户注册赠送积分活动 608662