Metabolomic Data Exploration and Analysis with the Human Metabolome Database

The Human Metabolome Database (HMDB) is a comprehensive, online, digital database designed to support the analysis and interpretation of metabolomic data acquired from human and/or mammalian metabolomic studies. This chapter covers three methods or protocols pertinent to using the HMDB: (1) understanding the general layout of the HMDB; (2) exploring the contents of a typical HMDB MetaboCard; and (3) an example of how HMDB can be used in a metabolomics study on human glioblastoma.