生化工程
合理设计
蛋白质工程
生物催化
功能(生物学)
蛋白质设计
计算机科学
工程设计过程
合成生物学
过程(计算)
生产(经济)
酶
工程类
计算生物学
纳米技术
化学
生物化学
生物
蛋白质结构
材料科学
细胞生物学
催化作用
操作系统
宏观经济学
离子液体
经济
机械工程
作者
Tanatarov Dinmukhamed,Ziyang Huang,Yanfeng Liu,Xueqin Lv,Jianghua Li,Guocheng Du,Long Liu
标识
DOI:10.1007/s43393-020-00005-9
摘要
Enzymes are considered as functional proteins, also known as biocatalysts, which are required for normal cellular function. The commercial production of enzymes with distinct features always remains the top priority of researchers for industrial biocatalysis. However, the insufficient yield, low stability, low activity, formation of by-products, complex purification process, and many other factors are challenging for the enzyme production industry. Novel advancements in protein engineering, especially the combination of multidisciplinary techniques including post-translational enzyme modification, structured assisted protein tailoring and computational modeling approaches, open a new horizon to more efficient production of biocatalysts. In this review, we focus on the development process of enzyme modification design strategies, such as rational design, semi-rational design and de novo design. Selected examples for each strategy, which are particularly useful for novel enzyme design, are given. We hope this review is helpful for the development of biocatalysts for industrial application.
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