生物
小RNA
拟南芥
RNA剪接
计算生物学
阿尔戈瑙特
竞争性内源性RNA
拟南芥
遗传学
核糖核酸
基因
RNA干扰
长非编码RNA
突变体
作者
Érika Frydrych Capelari,Guilherme Cordenonsi da Fonseca,Frank Guzmán,Rogério Margis
出处
期刊:Plants
[Multidisciplinary Digital Publishing Institute]
日期:2019-08-26
卷期号:8 (9): 302-302
被引量:28
标识
DOI:10.3390/plants8090302
摘要
Competing endogenous RNAs (ceRNAs) are natural transcripts that can act as endogenous sponges of microRNAs (miRNAs), modulating miRNA action upon target mRNAs. Circular RNAs (circRNAs) are one among the various classes of ceRNAs. They are produced from a process called back-splicing and have been identified in many eukaryotes. In plants, their effective action as a miRNA sponge was not yet demonstrated. To address this question, public mRNAseq data from Argonaute-immunoprecipitation libraries (AGO-IP) of Arabidopsis thaliana flowers were used in association with a bioinformatics comparative multi-method to identify putative circular RNAs. A total of 27,812 circRNAs, with at least two reads at the back-splicing junction, were identified. Further analyses were used to select those circRNAs with potential miRNAs binding sites. As AGO forms a ternary complex with miRNA and target mRNA, targets count in AGO-IP and input libraries were compared, demonstrating that mRNA targets of these miRNAs are enriched in AGO-IP libraries. Through this work, five circRNAs that may function as miRNA sponges were identified and one of them were validated by PCR and sequencing. Our findings indicate that this post-transcriptional regulation can also occur in plants.
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