Platinum Nanozyme-Triggered Pressure-Based Immunoassay Using a Three-Dimensional Polypyrrole Foam-Based Flexible Pressure Sensor

This work describes a novel and portable pressure-based point-of-care (POC) testing strategy for the sensitive and rapid detection of carcinoembryonic antigen (CEA) via a flexible pressure sensor constructed by three-dimensional (3D) polypyrrole (PPy) foam. Initially, platinum nanoparticles (PtNPs) were conjugated to the detection antibodies, which were used to form sandwich-type immunocomplexes with targets and capture antibodies in the reaction cell. Then, the carried PtNPs catalyzed the dissociation of hydrogen peroxide (H2O2) for the generation of oxygen (O2) in a sealed device, translating the biomolecule recognition event into gas pressure. With the increase of pressure, a flexible pressure sensor with 3D polypyrrole foam as the sensing layer was used to sensitively monitor the pressure variations in this system. Thus, the concentration of the target could be quantitatively determined by the pressure response. Under optimal conditions, the pressure-based immunosensor showed good sensing performance for CEA in the dynamic working range from 0.2 to 60 ng/mL with a detection limit of 0.13 ng/mL. The reproducibility, specificity, and accuracy compared with commercial enzyme-linked immunosorbent assay (ELISA) kit were also acceptable. Therefore, this work provides a promising approach for developing portable and sensitive POC testing in the future.