Neutrophil granulocytes promote flow stagnation due to dynamic capillary stalls following experimental stroke

活体显微镜检查 缺血 转基因小鼠 大脑中动脉 渗透(HVAC) 病理 脑缺血 闭塞 体内 医学 内皮干细胞 粒细胞 内科学 免疫学 转基因 心脏病学 炎症 冲程(发动机) 生物 微循环 体外 材料科学 物理 复合材料 生物技术 基因 热力学 生物化学
作者
Leoni Rolfes,Monika Riek‐Burchardt,Marc Pawlitzki,Jens Minnerup,Stefanie Böck,Mariella Schmidt,Sven G. Meuth,Matthias Gunzer,Jens Neumann
出处
期刊:Brain Behavior and Immunity [Elsevier BV]
卷期号:93: 322-330 被引量:27
标识
DOI:10.1016/j.bbi.2021.01.011
摘要

Flow stagnation of peri-ischemic capillaries due to dynamic leukocyte stalls has been described to be a contributor to ongoing penumbral injury in transient brain ischemia, but has not been investigated in permanent experimental stroke so far. Moreover, it is discussed that obstructing neutrophils are involved in this process; however, their contribution has not yet been proven. Here, we characterize the dynamics of neutrophil granulocytes in two models of permanent stroke (photothrombosis and permanent middle cerebral artery occlusion) using intravital two-photon fluorescence microscopy. Different to previous studies on LysM-eGFP+ cells we additionally apply a transgenic mouse model with tdTomato-expressing neutrophils to avoid interference from additional immune cell subsets. We identify repetitively occurring capillary stalls of varying duration promoted by neutrophils in both models of permanent cerebral ischemia, validating the suitability of our new transgenic mouse model in determining neutrophil occlusion formation in vivo. Flow cytometric analysis of peripheral blood (PB) and brain tissue from mice subjected to photothrombosis reveal an increase in the total proportion of neutrophils, with selective upregulation of endothelial adherence markers in the PB. In conclusion, the dynamic microcirculatory stall phenomenon that is described after transient ischemia followed by reperfusion also occurs after permanent small- or large-vessel stroke and is clearly attributable to neutrophils.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
PDF的下载单位、IP信息已删除 (2025-6-4)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
1秒前
JJ发布了新的文献求助10
1秒前
王子安完成签到,获得积分10
2秒前
橙色小人发布了新的文献求助10
3秒前
cyan发布了新的文献求助30
5秒前
6秒前
天天快乐应助喜喵喵采纳,获得10
6秒前
shinysparrow应助dream采纳,获得200
6秒前
8秒前
KevenDing完成签到,获得积分10
10秒前
wulalala发布了新的文献求助30
11秒前
CipherSage应助loski采纳,获得10
11秒前
我是老大应助loski采纳,获得10
11秒前
完美世界应助loski采纳,获得10
12秒前
李健应助loski采纳,获得10
12秒前
欣喜沛芹发布了新的文献求助10
14秒前
可爱的函函应助橙色小人采纳,获得10
15秒前
ED应助多发论文采纳,获得10
17秒前
17秒前
量子星尘发布了新的文献求助10
17秒前
Owen应助机智思真采纳,获得10
18秒前
传奇3应助loski采纳,获得10
19秒前
万能图书馆应助自觉的凛采纳,获得10
21秒前
25秒前
积极幻桃应助ssjjzhou采纳,获得10
26秒前
讨厌科研完成签到,获得积分10
27秒前
30秒前
Xw发布了新的文献求助10
32秒前
33秒前
失眠的霸完成签到,获得积分10
34秒前
ChatGPT发布了新的文献求助10
35秒前
37秒前
多发论文完成签到,获得积分20
38秒前
38秒前
39秒前
su发布了新的文献求助10
39秒前
Xw关闭了Xw文献求助
39秒前
大个应助fjm采纳,获得10
42秒前
而为发布了新的文献求助30
42秒前
机智思真发布了新的文献求助10
42秒前
高分求助中
A new approach to the extrapolation of accelerated life test data 1000
ACSM’s Guidelines for Exercise Testing and Prescription, 12th edition 500
‘Unruly’ Children: Historical Fieldnotes and Learning Morality in a Taiwan Village (New Departures in Anthropology) 400
Indomethacinのヒトにおける経皮吸収 400
Phylogenetic study of the order Polydesmida (Myriapoda: Diplopoda) 370
基于可调谐半导体激光吸收光谱技术泄漏气体检测系统的研究 350
Robot-supported joining of reinforcement textiles with one-sided sewing heads 320
热门求助领域 (近24小时)
化学 材料科学 医学 生物 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 遗传学 基因 物理化学 催化作用 冶金 细胞生物学 免疫学
热门帖子
关注 科研通微信公众号,转发送积分 3989378
求助须知:如何正确求助?哪些是违规求助? 3531442
关于积分的说明 11254002
捐赠科研通 3270126
什么是DOI,文献DOI怎么找? 1804887
邀请新用户注册赠送积分活动 882087
科研通“疑难数据库(出版商)”最低求助积分说明 809173