热带假丝酵母
生物膜
白色念珠菌
微生物学
粘附
白色体
生物
酵母
结晶紫
化学
细菌
生物化学
遗传学
有机化学
作者
Manjula Weerasekera,Gayan Kanchana Wijesinghe,Thilini A Jayarathna,T.D.C.P. Gunasekara,Neluka Fernando,Nilwala Kottegoda,LP Samaranayake
出处
期刊:Memorias Do Instituto Oswaldo Cruz
[SciELO]
日期:2016-11-01
卷期号:111 (11): 697-702
被引量:76
标识
DOI:10.1590/0074-02760160294
摘要
As there are sparse data on the impact of growth media on the phenomenon of biofilm development for Candida we evaluated the efficacy of three culture media on growth, adhesion and biofilm formation of two pathogenic yeasts, Candida albicans and Candida tropicalis. The planktonic phase yeast growth, either as monocultures or mixed cultures, in sabouraud dextrose broth (SDB), yeast nitrogen base (YNB), and RPMI 1640 was compared, and adhesion as well as biofilm formation were monitored using MTT and crystal violet (CV) assays and scanning electron microscopy. Planktonic cells of C. albicans, C. tropicalis and their 1:1 co-culture showed maximal growth in SDB. C. albicans/C. tropicalis adhesion was significantly facilitated in RPMI 1640 although the YNB elicited the maximum growth for C. tropicalis. Similarly, the biofilm growth was uniformly higher for both species in RPMI 1640, and C. tropicalis was the slower biofilm former in all three media. Scanning electron microscopy images tended to confirm the results of MTT and CV assay. Taken together, our data indicate that researchers should pay heed to the choice of laboratory culture media when comparing relative planktonic/biofilm growth of Candida. There is also a need for standardisation of biofilm development media so as to facilitate cross comparisons between laboratories.
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