大肠杆菌
脂多糖
化学
微生物学
生物化学
生物
基因
免疫学
作者
M. R. Sunayana,Manjula Reddy
出处
期刊:Bio-protocol
[Bio-Protocol]
日期:2015-01-01
卷期号:5 (24)
被引量:7
标识
DOI:10.21769/bioprotoc.1688
摘要
2-Keto-3-deoxy-octonate (KDO) IS an essential constituent of lipopolysaccharide (LPS) that forms the outermost leaflet of Gramnegative
ba([erial outer membrane. LPS is mainly composed of lipid A. 0 -antigen and a core oligosaccharide. Two molecules of
KDO are present per one molecule of LPS. A proper level of LPS is required to maintain the outer membrane Integrity and either
high or low levels of LPS are COXIC to the cell. Various methods are available for quantification of LPS; of these, determination of
KDO is a simple and accurate method and it can be estimated either directly from crude bacterial cell lysates or from purified
LPS by a simple colorimetric assay. Although this procedure can be theoretically used for any Gram-negative bacterium, we used
it routinely to measure KDO from celllysa tes of Escf1ericl7ia coli(E. colt) K12 strains.
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