藻蓝蛋白
荧光
化学
圆二色性
猝灭(荧光)
光化学
分子
结晶学
分析化学(期刊)
有机化学
生物
光学
蓝藻
遗传学
物理
细菌
作者
Songwen Tan,Xiaodong Tan,Zhenxing Chi,Dayin Zhang,Weiguo Li
出处
期刊:Chemosphere
[Elsevier]
日期:2018-02-01
卷期号:192: 171-177
被引量:23
标识
DOI:10.1016/j.chemosphere.2017.10.159
摘要
This work reports the influence of lead (Pb2+) on fluorescence characteristics and protein structure of phycocyanin molecules experimentally in vitro. The fluorescence intensity decreases with the increasing concentration of Pb2+ from 0 to 5 × 10-5 mol L-1, showing the fluorescence quenching of phycocyanin by Pb2+. The quenching process is suggested to be static regarding the calculation results and the experimental results of time-resolved fluorescence decay profiles. The synchronous fluorescence spectra show that the effect of Pb2+ on the Tyr residues of phycocyanin is more significant than the Trp residues. The forming of aggregation by the interaction of Pb2+ with phycocyanin molecules is suggested from the results of resonance light scattering spectra. The UV-Vis spectra of the protein skeleton of phycocyanin have a red-shift of about 10 nm with increasing the Pb2+ concentration from 0 to 5 × 10-5 mol L-1, indicating a change in the protein skeleton and its secondary structure. With the increasing Pb2+ concentration, the two negative peaks (209 nm and 218 nm) on circular dichroism spectra become smaller, showing a decrease of the α-helix structure. These results may give people a deeper understanding of that how the heavy metal (Pb2+) can affect the chemo-physical properties of phycocyanin.
科研通智能强力驱动
Strongly Powered by AbleSci AI