表型
脚手架
脂肪组织
材料科学
阿格里坎
细胞生物学
硫氧化物9
干细胞
细胞生长
组织工程
细胞分化
分子生物学
生物医学工程
基因表达
基因
生物
病理
生物化学
骨关节炎
医学
替代医学
关节软骨
作者
Xiaopeng Zhou,Yiqing Tao,Jingkai Wang,Dongyu Liu,Chengzhen Liang,Hao Li,Qixin Chen
摘要
Abstract Type II collagen is reported to have the capability of guiding adipose‐derived stem cells (ADSCs) to differentiate towards a nucleus pulposus (NP)‐like phenotype. So this study aimed to establish a three‐dimensional (3D) collagen scaffold using N,N‐(3‐dimethylaminopropyl)‐N′‐ethyl carbodiimide and N ‐hydroxysuccinimide (EDAC/NHS) to increase the efficiency of ADSC differentiation into NP‐like cells. Physical properties, such as porosity, biodegradation, and microstructure, and biological characteristics such as cytotoxicity, cell proliferation, and expression of relevant genes and proteins were measured to evaluate the efficacy of different scaffolds. Collagen scaffolds cross‐linked with EDAC/NHS exhibited higher biological stability, better spatial structure, and higher gene and protein expression of functional markers such as aggrecan, SOX9 and COL2 than those of other groups. Based on the results, freeze‐dried type II collagen cross‐linked with EDAC/NHS formed the best 3D scaffold, for inducing ADSC proliferation and differentiation toward a NP‐like phenotype. © 2016 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 104A: 1687–1693, 2016.
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