Comparison of three RNA extraction kits for detection of H1N1 influenza virus nucleic acids.

Aim To compare the efficiency of three RNA extraction methods for detection of H1N1 influenza(flu) viruses.Methods The efficiency of three RNA extraction methods including automated nucleic acid extraction method (Promega),Rneasy Mini Kit(Qiagen),RNA extraction Kit(H Biotech Co.Ltd in China)were compared and the specimens tested were the 10-1～10-4 serial dilution of twenty known H1N1 flu viruses throat washing samples collected from the patients with a cute respiratory tract infection.Nucleic acid of flu viruses RNA were extracted using each RNA extraction methods and detected by fluorescence real-time quantitative RT-PCR Kit (Daangene).Results The extraction eficiency of three RNA extraction methods had significant statistical difference (P0.05)in the detection of the dilution flu viruses.The extraction efficiency of the automated total nucleic acid extraction method (Promega)was the highest(relative 100%)and the Rneasy mini Kit(Qiagen),and RNA extraction Kit( H biological Co.Ltd in China)extraction methods were lower (relative 83.27% 、55.70% ,respectively).Conclusion The results indicate that it had significant difference between three RNA extraction methods,suggesting that the appropriate one should be choose for detection of flu viruses in clinical specimens.