大肠杆菌
生物化学
苹果酸脱氢酶
代谢工程
化学
柠檬酸合酶
乳酸脱氢酶
脱氢酶
苹果酸合酶
基因敲除
ATP合酶
酶
生物
基因
乙醛酸循环
异柠檬酸裂解酶
作者
Xianghao Wu,Mark A. Eiteman
摘要
Citramalic acid (citramalate) is a five carbon hydroxy-dicarboxylic acid and potential precursor for the production of methacrylic acid from renewable resources. We examined citramalate production in Escherichia coli expressing the citramalate synthase gene cimA. Although, knockouts in ldhA coding lactate dehydrogenase and glcB/aceB coding malate synthase did not benefit citramalate accumulation, knockouts in gltA coding citrate synthase, and ackA coding acetate kinase significantly increased citramalate accumulation compared to the control strain. A fed-batch process in a controlled fermenter using a glucose feed resulted in 46.5 g/L citramalate in 132 h with a yield of 0.63 g/g, over 75% of the theoretical maximum yield from glucose of 0.82 g/g. Biotechnol. Bioeng. 2016;113: 2670-2675. © 2016 Wiley Periodicals, Inc.
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