Expanding the range of CRISPR/Cas9-directed genome editing in soybean

基因组编辑 Cas9 清脆的 基因组 生物 计算生物学 遗传学 基因
作者
Reqing He,Pengxiang Zhang,Yuchuan Yan,Yu Chen,Liyun Jiang,Youlin Zhu,Dong Wang
出处
期刊:aBIOTECH [Springer Nature]
卷期号:3 (2): 89-98 被引量:2
标识
DOI:10.1007/s42994-021-00051-4
摘要

The CRISPR/Cas9 system has been widely applied for plant genome editing. The commonly used SpCas9 has been shown to rely on the protospacer adjacent motif (PAM) sequences in the canonical form NGG and non-canonical NAG. Although these PAM sequences are extensively distributed across plant genomes, a broader scope of PAM sequence is required to expand the range of genome editing. Here we report the adoption of three variant enzymes, xCas9, SpCas9-NG and XNG-Cas9, to produce targeted mutation in soybean. Sequencing results determined that xCas9 with the NGG and KGA (contains TGA and GGA) PAMs successfully induces genome editing in soybean genome. SpCas9-NG could recognize NGD (contains NGG, NGA and NGT), RGC (contains AGC and GGC), GAA and GAT PAM sites. In addition, XNG-Cas9 was observed to cleave soybean genomic regions with NGG, GAA and AGY (contains AGC and AGT) PAM. Moreover, off-target analyses on soybean editing events induced by SpCas9 and xCas9 indicated that two high-fidelity Cas9 variants including eSpCas9 (enhanced specificity SpCas9) and exCas9 (enhanced specificity xCas9) could improve the specificity of the GGA PAM sequence without reducing on-target editing efficiency. These findings significantly expand the scope of Cas9-mediated genome editing in soybean.The online version contains supplementary material available at 10.1007/s42994-021-00051-4.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
PDF的下载单位、IP信息已删除 (2025-6-4)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
盈滢应助nssm采纳,获得10
刚刚
刚刚
刚刚
bx完成签到,获得积分10
刚刚
進擊的巨人完成签到,获得积分10
1秒前
1秒前
Ann完成签到,获得积分10
1秒前
眼睛大雨筠应助Ye采纳,获得30
1秒前
张宇琪发布了新的文献求助10
1秒前
专注秋尽完成签到,获得积分10
1秒前
yzm发布了新的文献求助10
2秒前
万能图书馆应助gs采纳,获得10
2秒前
2秒前
2秒前
wll1091完成签到 ,获得积分10
3秒前
3秒前
zxm完成签到,获得积分10
3秒前
3秒前
若水完成签到,获得积分10
4秒前
4秒前
烟柳画桥完成签到,获得积分10
4秒前
wlj完成签到 ,获得积分10
4秒前
4秒前
陈秋发布了新的文献求助20
5秒前
chen发布了新的文献求助10
5秒前
5秒前
5秒前
佐佐的2xL完成签到,获得积分10
6秒前
6秒前
Carly发布了新的文献求助10
6秒前
华仔应助无昵称采纳,获得10
6秒前
LISHAN完成签到,获得积分10
7秒前
7秒前
甘乐发布了新的文献求助30
7秒前
7秒前
领导范儿应助Li采纳,获得10
8秒前
搞怪便当完成签到,获得积分10
8秒前
8秒前
yuxx发布了新的文献求助10
8秒前
Foliage发布了新的文献求助10
8秒前
高分求助中
A new approach to the extrapolation of accelerated life test data 1000
Cognitive Neuroscience: The Biology of the Mind 1000
Technical Brochure TB 814: LPIT applications in HV gas insulated switchgear 1000
ACSM’s Guidelines for Exercise Testing and Prescription, 12th edition 500
Nucleophilic substitution in azasydnone-modified dinitroanisoles 500
不知道标题是什么 500
A Preliminary Study on Correlation Between Independent Components of Facial Thermal Images and Subjective Assessment of Chronic Stress 500
热门求助领域 (近24小时)
化学 材料科学 医学 生物 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 遗传学 基因 物理化学 催化作用 冶金 细胞生物学 免疫学
热门帖子
关注 科研通微信公众号,转发送积分 3968964
求助须知:如何正确求助?哪些是违规求助? 3513877
关于积分的说明 11170569
捐赠科研通 3249201
什么是DOI,文献DOI怎么找? 1794692
邀请新用户注册赠送积分活动 875297
科研通“疑难数据库(出版商)”最低求助积分说明 804755