Clinical trials of new culture media for staphylococcus isolation

A comparative analysis of the quality of the developed nutrient media, Baird-Parker dry agar base and Vogel-Johnson dry agar Base and foreign analogues, was done based on results of clinical trials. The tested media were qualified by the main biological parameters, such as sensitivity, growth rate, and differentiating and inhibiting properties. The evaluation of statistical reliability of the results of trials of clinical samples was evaluated taking into account the number of parallel studies and the number of matches of the results of studies conducted by different performers. 116 clinical samples of received by a laboratory of the testing laboratory center for research from hospital no.164 over the period of clinical trials were analyzed. 46 cultures of potential pathogens were isolated when culturing on test and control media: S. aureus -35; S. epidermidis-6; S. saprophyticus - 5. Lecithinase activity on the medium “Baird-Parker dry agar Base” and mannitol fermentation on the medium “Vogel-Johnson dry agar Base” in the preliminary phenotypic test allow the isolation and differentiation of clinical isolates of S. aureus from S. epidermidis, and S. saprophyticus.