流式细胞术
细胞周期
细胞生物学
细胞分裂
计算生物学
生物
计算机科学
细胞
免疫学
遗传学
作者
Anusree Nair,Sonal M. Manohar
出处
期刊:Bioanalysis
[Future Science Ltd]
日期:2021-10-04
卷期号:13 (21): 1627-1644
被引量:21
标识
DOI:10.4155/bio-2021-0071
摘要
Cell cycle involves a series of changes that lead to cell growth and division. Cell cycle analysis is crucial to understand cellular responses to changing environmental conditions. Since its inception, flow cytometry has been particularly useful for cell cycle analysis at single cell level due to its speed and precision. Previously, flow cytometric cell cycle analysis relied solely on the measurement of cellular DNA content. Later, methods were developed for multiparametric analysis. This review explains the journey of flow cytometry to understand different molecular and cellular events underlying cell cycle using various protocols. Recent advances in the field that overcome the shortcomings of traditional flow cytometry and expand its scope for cell cycle studies are also discussed.
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