化学
阿布茨
食品科学
鼠李糖
半乳糖
氧化应激
发酵乳杆菌
生物化学
抗氧化剂
螯合作用
益生菌
DPPH
乳酸
细菌
有机化学
生物
植物乳杆菌
遗传学
作者
Shalaka Bhawal,Ankita Kumari,Suman Kapila,Rajeev Kapila
标识
DOI:10.1021/acs.jafc.1c03182
摘要
This study investigated the physicochemical characteristics and antioxidative role of novel acidic cell-bound exopolysaccharide (EPS-b) from probiotic Limosilactobacillus fermentum (MTCC 5898) and gained an insight into the structure–function relationship. The physicochemical analysis of EPS-b isolated by ultrasonication method revealed a heteropolysaccharide molecule with an average MW of 96.97 kDa composed of glucose and galactose subunits present in random-coiled conformation. Fourier transform infrared (FTIR) and nuclear magnetic resonance (NMR) analyses further supported the observation and indicated the presence of α-(1 → 6) linkages. The analyses implicated the significant influence of structural features on the antioxidative activity of EPS-b by showing remarkable ABTS scavenging, reducing, and metal chelating potential with increasing concentration. Besides, the EPS-b by its scavenging potential also maintained the oxidative balance in the Caco-2 cells under oxidative stress and preserved the cellular antioxidative defense system (CAT, GPx, SOD, HO1, and GCLC) at the basal level.
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