Protective effects of Coridius chinensis extracts on rat reproductive damage induced by manganese

Manganese (Mn2+ ) is an environmental pollutant, and testis is one of the main target organs. Coridius chinensis (C. chinensis), a traditional Chinese medicine, has been shown widely used in treating various kinds of pain, nephropathy and erectile dysfunction. In our recent study, we found that Mn2+ exposure caused testicular injury could be rescued in part by the antioxidant activity of C. chinensis extracts (CcE). However, there is dearth of extensive knowledge on the therapeutic effects of C. chinensis on manganese-induced reproductive toxicity. In the present study, Sprague-Dawley (SD) rats were administered manganese chloride alone or co-treated with CcE for 30 consecutive days. Results indicated that C. chinensis mediated suppression of spermatogenic dysfunction, and the number of apoptotic cells was significantly decreased in CcE-treated groups. Furthermore, the disintegrated testicular ultrastructural structure caused by Mn2+ was partially repaired in CcE-treated groups. C. chinensis significantly inhibited Mn2+ -induced decline in biomarkers of blood-testis barrier (BTB) including occludin, claudin1, zonula occludens-1 and junctional adhesion molecule 1, whereas it decreased the expression of focal adhesion kinase (FAK) and c-Src. This study demonstrated that c-Src and FAK might be involved in the repair of Mn2+ -induced testicular injury by C. chinensis, but further research is needed.