Application of visible light-based projection stereolithography for live cell-scaffold fabrication with designed architecture

立体光刻 材料科学 组织工程 制作 脚手架 光致聚合物 聚乙二醇 生物医学工程 PSL公司 纳米技术 化学工程 复合材料 聚合物 单体 医学 替代医学 几何学 数学 病理 工程类
作者
Hang Lin,Dongning Zhang,Peter G. Alexander,Guang Yang,Jian Tan,Allen C. Cheng,Rocky S. Tuan
出处
期刊:Biomaterials [Elsevier]
卷期号:34 (2): 331-339 被引量:338
标识
DOI:10.1016/j.biomaterials.2012.09.048
摘要

One-step scaffold fabrication with live cell incorporation is a highly desirable technology for tissue engineering and regeneration. Projection stereolithography (PSL) represents a promising method owing to its fine resolution, high fabrication speed and computer-aided design (CAD) capabilities. However, the majority of current protocols utilize water-insoluble photoinitiators that are incompatible with live cell-fabrication, and ultraviolet (UV) light that is damaging to the cellular DNA. We report here the development of a visible light-based PSL system (VL-PSL), using lithium phenyl-2,4,6-trimethylbenzoylphosphinate (LAP) as the initiator and polyethylene glycol diacrylate (PEGDA) as the monomer, to produce hydrogel scaffolds with specific shapes and internal architectures. Furthermore, live human adipose-derived stem cells (hADSCs) were suspended in PEGDA/LAP solution during the PSL process, and were successfully incorporated within the fabricated hydrogel scaffolds. hADSCs in PEG scaffolds showed high viability (>90%) for up to 7 days after fabrication as revealed by Live/Dead staining. Scaffolds with porous internal architecture retained higher cell viability and activity than solid scaffolds, likely due to increased oxygen and nutrients exchange into the interior of the scaffolds. The VL-PSL should be applicable as an efficient and effective tissue engineering technology for point-of-care tissue repair in the clinic.
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