Switching between O‐ and C‐Glycosyltransferase through Exchange of Active‐Site Motifs

Mechanistic implications: Distinct active-site motifs in plant aryl glucosyltransferases of the GT-1 family differentiate between C- and O-glycosylation activity on a phloretin acceptor. In the implicated protein design principle the exchange of active-site motifs results in reversible switch between C/O-glycoside specificity. The proposed mechanism of the C-glycosyltransferase involves direct nucleophilic displacement at the anomeric carbon. Detailed facts of importance to specialist readers are published as "Supporting Information". Such documents are peer-reviewed, but not copy-edited or typeset. They are made available as submitted by the authors. Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.