电子传输链
细胞色素c
生物物理学
电子转移
碳纳米管
线粒体
细胞内
血红素
化学
细胞器
生物化学
材料科学
纳米技术
光化学
生物
酶
作者
Xiaowei Ma,Lihua Zhang,Lirong Wang,Xue Xue,Jihong Sun,Yan Wu,Guozhang Zou,Xia Wu,Paul Wang,Wayne G. Wamer,Jun‐Jie Yin,Kaiyuan Zheng,Xing‐Jie Liang
出处
期刊:ACS Nano
[American Chemical Society]
日期:2012-11-29
卷期号:6 (12): 10486-10496
被引量:88
摘要
Single-walled carbon nanotubes (SWCNTs) are broadly used for various biomedical applications such as drug delivery, in vivo imaging, and cancer photothermal therapy due to their unique physiochemical properties. However, once they enter the cells, the effects of SWCNTs on the intracellular organelles and macromolecules are not comprehensively understood. Cytochrome c (Cyt c), as a key component of the electron transport chain in mitochondria, plays an essential role in cellular energy consumption, growth, and differentiation. In this study, we found the mitochondrial membrane potential and mitochondrial oxygen uptake were greatly decreased in human epithelial KB cells treated with SWCNTs, which accompanies the reduction of Cyt c. SWCNTs deoxidized Cyt c in a pH-dependent manner, as evidenced by the appearance of a 550 nm characteristic absorption peak, the intensity of which increased as the pH increased. Circular dichroism measurement confirmed the pH-dependent conformational change, which facilitated closer association of SWCNTs with the heme pocket of Cyt c and thus expedited the reduction of Cyt c. The electron transfer of Cyt c is also disturbed by SWCNTs, as measured with electron spin resonance spectroscopy. In conclusion, the redox activity of Cyt c was affected by SWCNTs treatment due to attenuated electron transfer and conformational change of Cyt c, which consequently changed mitochondrial respiration of SWCNTs-treated cells. This work is significant to SWCNTs research because it provides a novel understanding of SWCNTs' disruption of mitochondria function and has important implications for biomedical applications of SWCNTs.
科研通智能强力驱动
Strongly Powered by AbleSci AI