诱导多能干细胞
生物
SOX2
KLF4公司
体细胞
脐带血
林28
干细胞
质粒
细胞生物学
免疫学
胚胎干细胞
遗传学
基因
作者
Keisuke Okita,Tatsuya Yamakawa,Yukiko Matsumura,Yoshiko Sato,Naoki Amano,Akira Watanabe,Naoki Goshima,Shinya Yamanaka
出处
期刊:Stem Cells
[Wiley]
日期:2013-02-25
卷期号:31 (3): 458-466
被引量:642
摘要
Abstract The generation of induced pluripotent stem cells (iPSCs) provides the opportunity to use patient-specific somatic cells, which are a valuable source for disease modeling and drug discovery. To promote research involving these cells, it is important to make iPSCs from easily accessible and less invasive tissues, like blood. We have recently reported the efficient generation of human iPSCs from adult fibroblasts using a combination of plasmids encoding OCT3/4, SOX2, KLF4, L-MYC, LIN28, and shRNA for TP53. We herein report a modified protocol enabling efficient iPSC induction from CD34+ cord blood cells and from peripheral blood isolated from healthy donors using these plasmid vectors. The original plasmid mixture could induce iPSCs; however, the efficiency was low. The addition of EBNA1, an essential factor for episomal amplification of the vectors, by an extra plasmid greatly increased the efficiency of iPSC induction, especially when the induction was performed from αβT cells. This improvement enabled the establishment of blood-derived iPSCs from seven healthy donors ranging in age from their 20s to their 60s. This induction method will be useful for the derivation of patient-specific integration-free iPSCs and would also be applicable to the generation of clinical-grade iPSCs in the future.
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