自愈水凝胶
明胶
微流控
机械生物学
芯片上器官
生物医学工程
生物物理学
纳米技术
化学
旁分泌信号
剪应力
细胞生物学
材料科学
生物
生物化学
医学
受体
有机化学
复合材料
作者
Michelle B. Chen,Suthan Srigunapalan,Aaron R. Wheeler,Craig A. Simmons
出处
期刊:Lab on a Chip
[The Royal Society of Chemistry]
日期:2013-01-01
卷期号:13 (13): 2591-2591
被引量:156
摘要
The cardiovascular system is particularly well-suited to modelling with microfluidic technologies, and much progress has been made to create microfluidic devices that mimic the microvasculature. In contrast, microfluidic platforms that model larger blood vessels and heart valves are lacking, despite the clear potential benefits of improved physiological relevance and enhanced throughput over traditional cell culture technologies. To address this need, we developed a bilayer membrane microfluidic device to model the vascular/valvular three-dimensional environment. Key features of the platform include physiologically-relevant spatial arrangement of multiple cell types, fluid flow over an endothelial monolayer, a porous membrane that permits heterotypic cell interactions while maintaining cell compartmentalization, and a photopolymerizable gelatin methacrylate (gel-MA) hydrogel as a physiologically-relevant subendothelial 3D matrix. Processing guidelines were defined for successful in-channel polymerization of gel-MA hydrogels that were mechanically stable, had physiologically-relevant elastic moduli of 2–30 kPa, and supported over 80% primary cell viability for at least four days in culture. The platform was applied to investigate shear stress-regulated paracrine interactions between valvular endothelial cells and valvular interstitial cells. The presence of endothelial cells significantly suppressed interstitial cell pathological differentiation to α-smooth muscle actin-positive myofibroblasts, an effect that was enhanced when the endothelium was exposed to flow-induced shear stress. We expect this versatile organ-on-a-chip platform to have broad utility for mechanistic vascular and valvular biology studies and to be useful for drug screening in physiologically-relevant 3D cardiovascular microenvironments.
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