Applying silica gel column chromatography purify resveratrol from extracts of Morus alba L. Leaf

The resveratrol contained in mulberry leaves has manifold health care functions. In the experiment, resveratrol was extracted from mulberry leaf using a reflux extraction method of the organic solvent, then resveratrol was purified by silica gel column chromatography and determined by high performance liquid chromatography after mulberry leaf was extracted, filtered, and vacuum concentrated. The optimum extraction conditions were as follows: material quality concentration: 1:13, extraction temperature: 60°C, extraction time: 105 min, and concentration of ethanol: 80%. At these conditions, the yield of resveratrol was 0.0225%. Through the purity tests, mix chloroform and methanol in the ratio of 10:1 as the eluting solvent, the purified resveratrol by the silica gel column chromatography can be advanced from 0.8 to 99.3%.   Key words: Resveratrol, mulberry leaf, extraction technology, silica gel column chromatography, high performance liquid chromatography (HPLC).