三乙氧基硅烷
硅烷化
生物分子
硅烷
材料科学
傅里叶变换红外光谱
戊二醛
硅
表面改性
基质(水族馆)
图层(电子)
化学工程
椭圆偏振法
硅烷
化学
纳米技术
色谱法
薄膜
冶金
复合材料
工程类
地质学
海洋学
作者
Naga Siva Kumar Gunda,Minashree Singh,Lana Norman,Kamaljit Kaur,Sushanta K. Mitra
标识
DOI:10.1016/j.apsusc.2014.03.130
摘要
In the present work, we developed and optimized a technique to produce a thin, stable silane layer on silicon substrate in a controlled environment using (3-aminopropyl)triethoxysilane (APTES). The effect of APTES concentration and silanization time on the formation of silane layer is studied using spectroscopic ellipsometry and Fourier transform infrared spectroscopy (FTIR). Biomolecules of interest are immobilized on optimized silane layer formed silicon substrates using glutaraldehyde linker. Surface analytical techniques such as ellipsometry, FTIR, contact angle measurement system, and atomic force microscopy are employed to characterize the bio-chemically modified silicon surfaces at each step of the biomolecule immobilization process. It is observed that a uniform, homogenous and highly dense layer of biomolecules are immobilized with optimized silane layer on the silicon substrate. The developed immobilization method is successfully implemented on different silicon substrates (flat and pillar). Also, different types of biomolecules such as anti-human IgG (rabbit monoclonal to human IgG), Listeria monocytogenes, myoglobin and dengue capture antibodies were successfully immobilized. Further, standard sandwich immunoassay (antibody–antigen–antibody) is employed on respective capture antibody coated silicon substrates. Fluorescence microscopy is used to detect the respective FITC tagged detection antibodies bound to the surface after immunoassay.
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