ROS induction within 4T1 breast cancer cells by CuS NPs as chemodynamic agent

Minimally invasive and highly precise cancer cell death is a goal, and nanoformulations that can respond to the unique TME hold promise. This article describes the development of a nanoparticles (NPs) for chemodynamic treatment (CDT) to combat the cancer. As a consequence, CuS NPs cores are used as Fenton-like catalysts, while Alginate (Alg) is used as a stabilizer and template for entrapping and stabilizing Cu NPs. Here, Alg coated CuS NPs (CuS@Alg NPs) was synthesized via a green and facile method. To promote biocompatibility, Alg is added to the system. The malignant cells are killed by generated toxic hydroxyl radicals (·OH) using CuS@Alg NPs as a Fenton-like process catalyst. The effectiveness of NPs was assessed by MTT assay, live/dead cells staining, and ROS generation within cancer cells. Results not only confirmed ROS generation ability of CuS@Alg NPs within cells, but also show the anticancer ability of them. By CDT, the created smart nanoscale system showed remarkable effectiveness for breast cancer suppression.