纳米点
细菌生长
抗菌活性
催化作用
激进的
氮气
核化学
化学
生物化学
细菌
生物
有机化学
遗传学
物理化学
作者
Huiping Wang,Qixiang Wang,Qiwei Wang,Wenjuan Dong,Yang Liu,Qin Hu,Xiuli Song,Shaomin Shuang,Chuan Dong,Xiaojuan Gong
标识
DOI:10.1016/j.jclepro.2023.137337
摘要
Bacterial infections have become a considerable obstacle to public health due to the emergence of antibiotic resistance. Herein, we rationally developed a novel and attractive nitrogen-doped carbon nanodots (N-CNDs) as efficient metal-free artificial nanozymes that synergistically and efficiently inhibit bacterial survival by inheriting the bacteriostatic ability of tea polyphenols and light-triggered oxidase-like catalytic activity. The excellent oxidase-mimicking activity exhibited by N-CNDs in the light was mediated by superoxide radicals (•O2−) with a high maximum reaction rate (1.59 μM⋅s−1) and a low Michaelis constant (0.421 mM). Furthermore, the constructed N-CNDs-based nanozyme was demonstrated to serve as a complex biomaterial platform for efficient sterilization by light-driven oxygen molecule generation of •O2−. Remarkably, the inherent antibacterial ability of N-CNDs can effectively inhibit both Gram-positive and Gram-negative bacteria, particularly against Gram-positive S. aureus at a minimum inhibitory concentration (MIC90) of 375 μg⋅mL−1. The inactivation rates of N-CNDs alone agsinst E. coli and S. aureus were 59.72% and 37.15%, respectively. However, the inactivation rates of E. coli and S. aureus by N-CNDs achieved by light were as high as 97.91% and 80.02%, respectively. Encouragingly, a two-way ANOVA revealed that the concentration of N-CNDs and the duration of light exposure had a synergistic effect on antibacterial behavior, which provided favorable conditions for the sustained eradication of exposed bacteria. This synergistic strategy for bacterial eradication based on a novel metal-free carbon-based nanozyme provides a powerful and feasible approach for bacterial contamination management in food safety and environmental protection.
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