胆囊癌
癌症研究
免疫印迹
细胞生长
裸鼠
MTT法
免疫组织化学
转移
荧光素酶
下调和上调
细胞迁移
生物
癌症
体外
分子生物学
化学
细胞培养
转染
免疫学
基因
生物化学
遗传学
作者
Cheng Zhao,Jian Zhang,Ziyi Yang,Liu-qing Shi,Shilei Liu,Lijia Pan,Ping Dong,Yi Zhang,Sheng Xiang,Yijun Shu,Jiawei Mei
出处
期刊:Phytomedicine
[Elsevier]
日期:2023-06-01
卷期号:114: 154785-154785
标识
DOI:10.1016/j.phymed.2023.154785
摘要
Gallbladder cancer (GBC) is the most aggressively malignant tumor in the bile duct system. The prognosis for patients with GBC is extremely poor. Ponicidin is a diterpenoid compound extracted and purified from the traditional Chinese herb Rabdosia rubescens, and showed promising anti-cancer effects in a variety of tumors. However, Ponicidin has not been investigated in GBC.CCK-8, colony formation assay and EdU-488 DNA synthesis assay were performed to investigate the effect of Ponicidin on GBC cells proliferation. Cell invasion and migration assays and wound-healing assay were used to explore the effect of Ponicidin on invasion and migration ability of GBC cells. mRNA-seq was adopted to explore the underlying mechanisms. Western blot and immunohistochemical staining were conducted to detect the protein level. CHIP assay and dual-luciferase assay were used to validate binding motif. Nude mouse model of GBC was used to assess the anti-tumor effect and safety of Ponicidin.Ponicidin inhibited the proliferation and cell invasion and migration of GBC cells in vitro. Moreover, Ponicidin exerted anti-tumor effects by down-regulating the expression of MAGEB2. Mechanically, Ponicidin upregulated the FOXO4 expression and promoted it to accumulate in nucleus to inhibit the transcript of MAGEB2. Furthermore, Ponicidin suppressed tumor growth in the nude mouse model of GBC with excellent safety.Ponicidin may be a promising agent for the treatment of GBC effectively and safely.
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