Covalent assembly-based two-photon fluorescent probes for in situ visualizing nitroreductase activities: from cancer cells to human cancer tissues

硝基还原酶 自体荧光 荧光 癌细胞 荧光团 化学 生物物理学 原位 癌症 体外 癌症生物标志物 结直肠癌 共价键 双光子激发显微术 生物化学 癌症研究 生物 遗传学 物理 有机化学 量子力学
作者
Chenfeng Xu,Zhongxi Huang,Jia Zhou,Wei Jiang,J Geng,Li Wang,Chibin Pu,Lin Li,Changmin Yu,Wei Huang
出处
期刊:Biosensors and Bioelectronics [Elsevier]
卷期号:267: 116768-116768 被引量:1
标识
DOI:10.1016/j.bios.2024.116768
摘要

Nitroreductase (NTR) is widely regarded as a biomarker whose enzymatic activity correlates with the degree of hypoxia in solid malignant tumors. Herein, we utilized 2-dimethylamino-7-hydroxynaphthalene as fluorophore linked diverse nitroaromatic groups to obtain four NTR-activatable two-photon fluorescent probes based on covalent assembly strategy. With the help of computer docking simulation and in vitro assay, the sulfonate-based probe XN3 was proved to be able to identify NTR activity with best performances in rapid response, outstanding specificity, and sensitivity in comparison with the other three probes. Furthermore, XN3 could detect the degree of hypoxia by monitoring NTR activity in kinds of cancer cells with remarkable signal-to-noise ratios. In cancer tissue sections of the breast and liver in mice, XN3 had the ability to differentiate between healthy and tumorous tissues, and possessed excellent fluorescence stability, high tissue penetration and low tissue autofluorescence. Finally, XN3 was successfully utilized for in situ visualizing NTR activities in human transverse colon and rectal cancer tissues, respectively. The findings suggested that XN3 could directly identify the boundary between cancer and normal tissues by monitoring NTR activities, which provides a new method for imaging diagnosis and intraoperative navigation of tumor tissue.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
大幅提高文件上传限制,最高150M (2024-4-1)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
充电宝应助Master采纳,获得10
1秒前
研友_VZG7GZ应助YY采纳,获得30
3秒前
4秒前
7秒前
7秒前
高挑的幼翠完成签到 ,获得积分10
8秒前
着急的白羊完成签到,获得积分10
8秒前
冷酷的向日葵完成签到,获得积分10
9秒前
10秒前
11秒前
CodeCraft应助秋蚓采纳,获得10
13秒前
锦瑟发布了新的文献求助10
13秒前
13秒前
斯文败类应助你好啊采纳,获得10
14秒前
开心发布了新的文献求助10
14秒前
16秒前
火星上盼山完成签到,获得积分10
17秒前
Master发布了新的文献求助10
19秒前
spss2005完成签到,获得积分20
20秒前
lql发布了新的文献求助10
20秒前
23秒前
luckyd完成签到 ,获得积分0
24秒前
斯文的道罡完成签到,获得积分10
24秒前
星辰大海应助小马到处跑采纳,获得10
25秒前
25秒前
spss2005发布了新的文献求助10
26秒前
27秒前
linpa完成签到 ,获得积分10
28秒前
柯仇天完成签到,获得积分10
29秒前
richardzhang1984完成签到 ,获得积分10
30秒前
Master完成签到,获得积分10
30秒前
李健应助远方的蓝风铃采纳,获得10
31秒前
曾建发布了新的文献求助10
32秒前
32秒前
iNk应助科研通管家采纳,获得20
33秒前
CodeCraft应助科研通管家采纳,获得10
33秒前
33秒前
wuwei发布了新的文献求助10
33秒前
cdu完成签到,获得积分10
35秒前
秋蚓完成签到,获得积分10
41秒前
高分求助中
Sustainability in Tides Chemistry 2800
Kinetics of the Esterification Between 2-[(4-hydroxybutoxy)carbonyl] Benzoic Acid with 1,4-Butanediol: Tetrabutyl Orthotitanate as Catalyst 1000
The Young builders of New china : the visit of the delegation of the WFDY to the Chinese People's Republic 1000
Rechtsphilosophie 1000
Bayesian Models of Cognition:Reverse Engineering the Mind 888
Handbook of Qualitative Cross-Cultural Research Methods 600
Very-high-order BVD Schemes Using β-variable THINC Method 568
热门求助领域 (近24小时)
化学 医学 生物 材料科学 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 基因 遗传学 催化作用 物理化学 免疫学 量子力学 细胞生物学
热门帖子
关注 科研通微信公众号,转发送积分 3137638
求助须知:如何正确求助?哪些是违规求助? 2788565
关于积分的说明 7787590
捐赠科研通 2444902
什么是DOI,文献DOI怎么找? 1300139
科研通“疑难数据库(出版商)”最低求助积分说明 625814
版权声明 601023