Brominated Flame Retardant HBCD and Artificial Light at Night Synergically Caused Visual Disorder and Sleep Difficulty in Zebrafish Larvae

Sleep difficulty is a widespread health concern exacerbated by factors such as light and chemical pollution. Artificial light at night (ALAN) can disrupt natural sleep-wake cycles, whereas chemical pollutants can impair sleep-related processes. The prevalence of ALAN increases the health risk of coexposure, yet it has not gained sufficient attention. Meanwhile, visual inputs are important for sleep regulation, especially the non-image-forming circadian visual system centered around melanopsin. This study evaluated the light perception ability and sleep performance of zebrafish larvae exposed to flame retardant hexabromocyclododecanes (HBCDs) at environmentally relevant concentrations (2.5 and 25 μg/L) and to cotreatment of HBCD and ALAN. HBCD induced a longer sleep latency of 34.59 min under 25 μg/L (