Recently, Raman spectroscopy has been developed rapidly and employed for food quality assurance and safety monitoring due to its distinctive advantages of water-insensitive, real-time, field-deployable, label-free, and fingerprinting-identification features as compared with traditional large-scale laboratory-based analytical techniques. This review article discusses the detection/measurement methods of Raman spectroscopy in food safety and quality assurance, including food-sample pretreatment methods, detection-platform design, data analysis, chemometrics, and Raman readers. In addition, this article highlights applications and advantages of Raman technologies in food-production chain, including plant growth, food processing, transportation, storage, and marketing.