酵母
单元格排序
流式细胞术
门控
酿酒酵母
细胞
分类
定向进化
表型
计算生物学
生物
细胞生物学
基因
计算机科学
遗传学
生物物理学
算法
突变体
作者
Xiaoli Pan,Matheus Oliveira de Souza,Francisco M Figueiras,Aric Huang,Bailey B. Banach,Jacy R. Wolfe,Azady Pirhanov,Bharat Madan,Brandon J. DeKosky
出处
期刊:Protein Engineering Design & Selection
[Oxford University Press]
日期:2024-12-12
标识
DOI:10.1093/protein/gzae018
摘要
Yeast display is a widely used technology in antibody discovery and protein engineering. The cell size of yeast enables fluorescence-activated cell sorting (FACS) to precisely screen gene libraries, including for multi-parameter selection of protein phenotypes. However, yeast cells show a broader size distribution than mammalian cells that complicates single-cell gate determination for FACS. In this report, we analyze several yeast display gating options in detail and present an optimized strategy to select single yeast cells via flow cytometry. These data reveal optimized single-cell gating strategies to support robust and high-efficiency yeast display studies.
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